Preparation of melanin from silkworm wastes and studying its physical and chemical characteristics
Abstract
The paper presents a scheme for preparation of melanin from silkworm wastes and studies of its physicochemical characteristics and individual composition. The optimal conditions for the isolation of melanin from silkworm wastes, providing the maximum yield of the target product, have been established. It has been established that the main substance of melanin isolated from silkworm wastes is 5,6-dioxiindole homopolymers. The black dry substance, the content of the target product with an average molecular weight of 58300 ± 112 and a density of 1.0 g/cm3 is 96-98 %. The maximum molar absorption is observed at a wavelength of 555 nm, it is well soluble in water, oils and organic solvents.
References
Kuznetsova O. Yu. Sorption capacity of chaga melanins // Bulletin of Kazan Technological University. - 2013. - V. 16. - No. 23. - P. 136-138. (in Russian)
Nosov A.I., Sysoeva M.A., Grevtsev V.A., Khalitov F.G. Investigation of the physicochemical properties of chromogenic complexes of flat and edged tinder // Chemistry of plant raw materials. - 2013. - No. 3. - P.195-200. (in Russian)
Veleshko I.E., Veleshko A.N., Rumyantseva E.V., Rozanov K.V. et al. Sorption of radionuclides by chitin-melanin by the glucan complex Mikoton // Chemistry of plant raw materials. - 2011. - No. 4. - P. 39-48. (in Russian)
Sysoeva M.A. Highly active antioxidants based on the fungus Inonotus obliquus: abstract of dissertation of doctor of chemical sciences. Kazan, 2009. 32 p. (in Russian)
Sysoeva M.A., Ivanova G.A., Zakharova L.Ya. The affect of the hydrophobic component of chaga melanin on its structure and antioxidant activity // Bulletin of Kazan Technological University. - 2010. - No. 9. - P. 525-600. (in Russian)
Baraboy V. A. Structure, biosynthesis of melanins, their biological role and application prospects // Successes of modern biol. - 2001. - V. 121. - No. 1. - P. 36-46. (in Russian)
Gracheva N. V., Zheltobryukhov V. F. A method for preparation of melanin from sunflower husks and the study of its antioxidant activity // Bulletin of Kazan Technological University. - 2016. - V. 19. - No. 15. - P. 154-157. (in Russian)
Ogarkov B.N., Samusenok L.V. A method for preparation of a pigment-dye from plant materials // Patent RU. - T. 2215761 (2003). (in Russian)
Gracheva N. V., Kartushina Yu. N., Danilova M. A., Golovanchikov A. B., Zheltobryukhov V. F. A method for preparation of a melanoid antioxidant from sunflower husk // RU Patent. - T. 2578037 (2015). (in Russian)
Burmasova M. A., Sysoeva M. A. Composition of substances of butanol extract from chaga melanin // Chemistry of plant raw materials. - 2012. - No. 1. - P. 149–152. (in Russian)
Kuzayev A.I. et al. Determination of molecular weights of tetrafluoroethylene telomers by gel permeation chromatography // High molecular weight compounds. Series A. - 2009. - V. 51. - No. 7. - P. 1122-1127. (in Russian)
Tashmatova R.V., Kalanova M.B. Certification of melanin isolated from silkworm waste // Materials of the VI Republican Scientific and Practical Conference “Problems and Prospects of Chemistry and Chemistry of Goods” (with the participation of foreign scientists) - Andijon, 2019.- P. 47-48.
Mukhamadiev N. Q. et al. Optimization of separation on the basis of UNIFAC parameters and evaluation of the composition of the stationary phase in gas-liquid chromatography // Chromatographia. - 2003. - V. 57. - No. 11-12. - P. 831-833.
Fazlieva N.T., Muhamadiev N.Q., Sayitkulov Sh.M. Study Iodine Amount in Content of Some Medicinal Plants by Ion Chromatography // International Journal of Chemical and Physical Sciences. - 2014. - V.3. - N 6. - P. 91-95.
Muhamadiev N.Q., Fazlieva N.T., Sayitkulov Sh. M. Optimization of separation processes in chromatography using LIQUAC model // International Journal of Engineering Sciences & Research Technology. - 2015. - V.4. - N 1. - P. 663-666.
Fazlieva N.T., Muhamadiev N.Q., Sayitkulov Sh.M., Khalilov Q.F. The optimization algorithm of separation process in ion chromatography // International Journal of Engineering Sciences & Research Technology. - 2015. - V.4. - N 9. - P. 1-3.
