Association of Carbamoyl Phosphate Synthase-1 Polymorphism (RS1047891) with Primary Hypertension among Hypertensives in Ibadan South-West Nigeria
Keywords:
Nigeria, Polymorphism
Abstract
Carbamoyl phosphate synthase 1 (CPS1) is a key gene in the first step of urea cycle. A functional single nucleotide polymorphism C/A has been reported to be associated with high levels of homocysteine. Increase in the homocysteine levels result to increase in hypertension most especially among the aged. This study, thus investigated the association of CPS1 (rs1047891) with primary hypertension with reference to a global simulated data.
A total of 100 participants, 50 normotensives were matched with 50 hypertensives within 18-80 years of age in Ibadan, South-west Nigeria were studied. A total of 2000 simulated global data set was used having 382 as normotensives and 1618 as hypertensives. Blood samples of participants from Ibadan were taken and other clinical parameters such as age, sex weight, height, systolic blood pressure and diastolic blood pressure were determined. The participants were genotyped for one SNP variant (rs1047891) using polymerase chain reaction and enzymatic restriction. Statistical analysis was performed using Haploview 4.2, Plink and SPSS ® 20.0 software for windows and p-value set at p<0.05.
The minor allele frequency of rs1047891 of CPS1 gene was similar to that in the hapmap database among the Yoruba population. The distribution of rs1047891 of CPS1 gene and n4_3, n4_4, m4_0 and qr2_4 of the simulated global data set genotypes was consistent with the Hardy-Weinberg equilibrium (P>0.05). There was no statistically significant association (p>0.05) between the polymorphism of CPS1 gene and SNPs in the simulated global data set with hypertension.
References
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28. da Silva, A., do Carmo, J., Dubinion, J., & Hall, J. (2009). The role of the sympathetic nervous system in obesity-related hypertension. CurrentHypertension Reports, 11(3), 206-211.
29. Dankner, R., Chetrit, A., Lubin, F., Sela, B.(2004). Life-style habits and homocysteine levels in an elderly population. Aging Clin. Exp. Res. 16, 437–442.
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2. Adachi, H., Hirai, Y., Fujiura, Y., Matsuoka, H., Satoh, A., Imaizumi, T.(2002). Plasma homocysteine levels and atherosclerosis in Japan: epidemiological study by use of carotid ultrasonography. Stroke 33, 2177–2181.
3. Addo, J., Smeeth, L., Leon, D. (2007). Hypertension in sub-saharan Africa: a systematic review. Hypertension 50 (6): 1012-8.
4. Adeloye, D., Basquill, C., Aderemi, A., Thompson, J., Obi, F. (2015). An estimate of the prevalence of hypertension in Nigeria: a systematic review and meta-analysis. J Hypertens 33: 230–242. doi: 10.1097/HJH.0000000000000413.
5. Adeyemo, A., Gerry, N., Chen, G., Herbert, A., Doumatey, A., Huang, H., Zhou, J., Lashley, K.., Chen, Y., Christman, M., & Rotimi, C. (2009). A genome-wide association study of hypertension and blood pressure in African Americans. PLoS Genetics, 5, e1000564.
6. Afifah Binti Azam and Elena Aisha Binti Azizan (2018). Brief Overview of a Decade of Genome-Wide Association Studies on Primary Hypertension.International Journal of Endocrinology. Article ID 7259704, 14 pages.
7. Agbelusi, O. (2014). Development of a predictive model for survival of HIV/AIDS patients in south-western Nigeria, unpublishedMPhil Thesis, Obafemi Awolowo University, Ile-ife, Nigeria.
8. Amballi A., Ayinde A., Asaolu O., & Olabumyi O. (2022). Effect of Work Motivation on Job Performance Among Healthcare Providers in University College Hospital, Ibadan, Oyo State. Central Asian Journal of Medical and Natural Sciences. 03 (02): 219-233
9. Amrit Singh (2007). Hypertension. Primary Care Geriatrics (Fifth Edition), Pages 428-449.
10. Ana Isabel Martínez, Isabel Pérez-Arellano, Satu Pekkala, Belén Barcelona, Javier Cervera (2010). Genetic, structural and biochemical basis of carbamoyl phosphate synthetase 1 deficiency. Molecular Genetics and Metabolism 101: 311-323.
11. Ataklte, F., Erqou, S., Kaptoge, S., Taye, B., Echouffo-Tcheugui, J., Kengne, A. (2015). Burden of undiagnosed hypertension in sub-saharan Africa: a systematic review and meta-analysis. Hypertension;65:291–8. doi:10.1161/HYPERTENSIONAHA.114.04394.
12. AvinashKumar, Henry Palfrey, Rashmi Pathak, Philip Kadowtz, Thomas Gettys and Subramanyam Murthy, (2017). The metabolism and significance of homocysteine in nutrition and health.Nutr Metab; 14: 78.
13. Ayinde A., Asaolu O., & Alawal O. (2022). Birth Order and Exclusive Breastfeeding Practices among Mothers with Two or More Children. Central Asian Journal of Medical and Natural Sciences. 03 (02): 137-148
14. Bavishi, C., Goel, S., Messerli, F. (2016). Isolated systolic hypertension: An update after SPRINT. The American Journal of Medicine. 129(12): 1251- 1258.
15. Burton, P., Clayton, D., Cardon, L., Craddock, N., Deloukas, P., Duncanson, A., .Samani, N. (2007). Genome-wide association study of 14,000 cases of seven common diseases and 3,000 shared controls. Nature,447(7145), 661-678.
16. Butler, M.G. (2010). Genetics of hypertension. current status. J Med Liban; 58:175-8.
17. Calhoun, D., Bakir, S., Oparil, S. (2000). Etiology and pathogenesis of essential hypertension. In: Crawford MH, DiMarco JP, eds. Cardiology. London: Mosby International;3.1-3.10.
18. Cappuccio, F.P., Sally, M.K., Frank, B.M., Jacob, P.R., John, B.E. (2006). A community programme to reduce salt intake and blood pressure in Ghana. BMC Public Health.
19. Carey, R.M., Siragy, H.M. (2003). Newly recognized components of the renin-angiotensin system: potential roles in cardiovascular and renal regulation. Endocr Rev; 24:261-71.
20. Carretero, O., Oparil, S.(2000). Essential hypertension. Part I: definition and etiology. Circulation 101(3):329–335. doi: 10.1161/01.CIR.101.3.329.
21. Carrire, Orexin (2017). Stress and Central Cardiovascular Control. A Link with Hypertension? Neuroscience & Biobehavioral Reviews. 74: p. 376-392.
22. Chai-Ti, Tsai., Daniele Fallin, Fu-Tien., Chiang, Juey-Jen Hwang., Ling-Ping, Lai., Kuan-Lih, Hsu., Chuen-Den, Tseng., Chiau-Suong, Liau and Yung-Zu, Tseng. (2003). Angiotensinogen gene haplotype and hypertension: Interaction with ACE gene 1 allele. Hypertension,SCOPUS: 41(1), 9-15.
23. Chang, Y., Liu, X., Kim, J., Ikeda, M., Layton, M., Weder, A., Hunt, S. (2007). Multiple genes for essential-hypertension susceptibility on chromosome 1q. The American Journal of Human Genetics, 80(2), 253-264.
24. Chang, Y., Liu, X., Kin, J., Ikeda, M., Layton,M., Weder, A., Cooper, R., Kardia, S., Rao, D., Hunt, S., Luke, A., Boerwinkle, E., Chakravarti, A. (2007). Multiple genes for essential-hypertension susceptibility on chromosome 1q. Am. J. Hum. Genet. 80, 253–264.
25. Charles, L., Triscott, J and Dobbs B. (2017). Secondary Hypertension: Discovering the underlying Cause. Am Fam Physician. Oct 1; 96 (7): 453-461
26. Clara Appiagyei Nkyi (2016). Review of Hypertension in sub-Saharan Africa. Doi: 10.31191/afrijcmr.v1i1.8
27. Cornier, M., Despres, J., Davis, N., Grossniklaus, D., Klein, S., Lamarche, B., Lopez-Jimenez, F., Rao, G., Stonge, M., Towfighi, A., Poirier, P.(2011). Assessing adiposity. A scientific statement from the American Heart Association.
28. da Silva, A., do Carmo, J., Dubinion, J., & Hall, J. (2009). The role of the sympathetic nervous system in obesity-related hypertension. CurrentHypertension Reports, 11(3), 206-211.
29. Dankner, R., Chetrit, A., Lubin, F., Sela, B.(2004). Life-style habits and homocysteine levels in an elderly population. Aging Clin. Exp. Res. 16, 437–442.
30. David Guwatudde,Joan NankyaMutyoba,Robert Kalyesubula,Carien Laurence,Clement Adebamowo,IkeOluwapo Ajayi,Francis Bajunirwe,Marina Njelekela,Faraja S. Chiwanga,Todd Reid,Jimmy Volmink,Hans-Olov Adami,Michelle D. Holmes andShona Dalal(2015). The burden of hypertension in sub-Saharan Africa: a four-country cross sectional study.BMC Public Health15, Article number: 1211.
Published
2022-05-27
How to Cite
Kesinro , M. O., O , A. A., Asaolu, O., Adeoye, L. P., & Obembe, O. (2022). Association of Carbamoyl Phosphate Synthase-1 Polymorphism (RS1047891) with Primary Hypertension among Hypertensives in Ibadan South-West Nigeria. Central Asian Journal of Medical and Natural Science, 3(3), 246-261. https://doi.org/10.17605/cajmns.v3i3.772
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