Determination of microorganisms markers by the method GC-MS and efficacy evaluation of rhinosinusitis

  • Karabaev H.E. Tashkent Pediatric Medical Institut
  • Mamatova Sh.R. Tashkent Pediatric Medical Institut
  • Muhamadiev N.K. Samarkand State University
  • Naimova B.K. Samarkand State University
  • Berdimurodova F.P. Samarkand State Medical University
Keywords: children, early age, acute, sinusitis, ethereal oil, anise, chromatography, marker, quality, quantity, microflora

Abstract

The work is devoted to determination of microorganisms markers by the method GC-MS (gas chromography with ions mass detection) and efficacy evaluation of rhinosinusitis treatment. The microorganisms markers were revealed by the method GC-MS from nasal cavity, and, there were detected such microorganisms as: Streptococcus pneumoniae, Haemophilus influenzae Staphylococcus aureus, Streptococcus viridans. β-hemolytic streptococcus group A, Streptococcus mitis/oralis, Streptococcus sanguinis, Staphylococcus saprophyticus, Staphylococcus epidermidis, Streptococcus haemolyticus Gram (-) flora were typical for children of yearly age with acute rhinosinusitis which served as diagnostic criterion. Use of anise ethereal oil as antimicrobial, antiviral, antioxidant medicine led to restore microbial view in patients with acute rhinosinusitis and brought them to normal indices with efficacy 98 %.

References

Mamatova Sh.R., Karabaev H.E., Namahanov A.M. “Ultrasonic study rhinosinusitis at children of yearly age” “Journal of biomedicine and practice” Vol.6, no.2, Tashkent 2021, P. 68-63.

Karabaev H.E., Mamatova Sh.R.“Clinical case of orbital complication aftert rhinosinusitis in children of yearly age.” “Eurasian Bulletin” no.3,2020 Toshkent, P. 78-82

Karabaev H.E., Mamatova ShR., Abdurahmanov H.N. “Acute rhinosinusitis at children: up to date treatment in 2020” “Tibbiyotda yangi kun” 4 (32) 2020 Toshkent, P. 18-23

Anand V.K. Epidemiology and economic impact of rhinosinusitis // Ann Otol Rhinol Laryngol Suppl. 2004. Vol. 193. Р. 3–5.

Yanov U.K. and et al. Practical recommendations on antibacterial therapy of sinusitis (manual for doctors). Col., 2002 P.

Krukov A.I., Stoodoniy M.E., Artemyev M.E. and et al. Treatment of patients with rhinosinusitis: possibilities of conservative and operative influence // Medical Council, 2012, no. 11.

Lopatin A.S. Anti-biotic-therapy of acute inflammatory diseases for paranasal sinuses // Consilium mediсum. 2003. Vol. 05., no. 4. P. 1–8.

Ryazantsev S.V. Acute sinusitis. The approaches to therapy (methodical recommendations). M., 2003. P.16

Svistushkin V.M., Shevchik E.A. Acute rhinosinusitis–modern point of view on the problem //PMJ. – 2014. – Vol. 9. – P. 643-648.

Hadley J. A., Pfaller M. A. Oral beta-lactams in the treatment of acute bacterial rhinosinusitis // Diagn Microbiol Infect Dis. 2007. Vol. 57 (3 Suppl). Р. 47–54.

Lopatin A.S., Svistushkin V.M. Acute rhinosinusitis: etiology, pathogenesis, diagnosis and principles of treatment //Clinical recommendations. – 2009. – no. P .12.

Kolosov A.V., Guchev I. A., Krechikova O.I. Acute bacterial rhinosinusitis at military service men: etiology, sensitivity to antibiotics and efficacy of antimicrobial therapy // Clinical microbiology and antimicrobial chemotherapy. 2009, no. 11 (1). P. 14–21.

Otvagin I.V. Pharma-epidemiological and clinic-bacterial bases of use cephalosporines at patients with acute sinusitis: Synopsis of thesis, cand. of med. scien., Smolensk, 1998. P. 22.

Strachinskiy L.S., Tarasov A.A., Krukov A.I. with co-authors. The agents of acute bacterial rhinosinusitis. The results of multicentral microbiological researches of SSSR // Clinical microbiology and antimicrobial chemotherapy. 2005., no.7 (4). P. 337–349.

Chow A.W., Benninger M.S., Brook I. et al. IDSA clinical practice guideline for acute bacterial rhinosinusitis in children and adults // Clin Infect Dis. 2012; Vol. 54 (8). Р. 72–112.

Osipov GA, Verkhovtseva NV. Study of human microecology by mass spectrometry of microbial markers. Benef Microbes. 2011 Mar;2(1):63-78. doi: 10.3920/BM2010.0017

Yang Y, Misra BB, Liang L, et al. Integrated microbiome and metabolome analysis reveals a novel interplay between commensal bacteria and metabolites in colorectal cancer. Theranostics. 2019 May 31;9(14):4101-14. doi: 10.7150/thno.35186. eCollection 2019.

Ktsoyan ZA, Beloborodova NV, Sedrakyan AM, et al. Profiles of Microbial Fatty Acids in the Human Metabolome are DiseaseSpecific. Front Microbiol. 2011 Jan 20;1:148. doi: 10.3389/fmicb.2010.00148. eCollection 2010.

Fokkens W., Lund V., Mullol J. et al. European position paper on rhinosinusitis and nasal polyps 2012 (EP3OS) // Rhinology, 2012, vol. 50 (23), p. 1–299.

Zaplatnikov A.L. Topical decongestants in pediatric practice: safety and clinical efficacy // Pediatrics, 2006, no. 6, с. 69–75.

Tulupov D.A., Karpova E.P. About the role of nasal vascular constricting medicines in symptomatic treatment of acute sinusitis at children // Russian rhinology, 2011, no. 2. P. 50.

Kolesnikova M.B., Kilina A.V. Efficacy of use ethereal oils in prophylaxis of acute respiratory diseases at preschool children in organized offices //Bulletin on otorhinolaryngology, 2011, no.5. P. 51-54

Karpova E.P., Vagina Е. Е. Use inhalation therapy with ethereal oils in prophylaxis and complex treatment of children with acute rhinosinusitis//Pediatrician’s practice. – 2015. – no. 1. – P. 21-26.

Karpova E.P., Tulupov D.A. About safety of use nasal vascular-constricting drugs in pediatric practice // Russian rhinology, 2014, no.1, P. 12–14.

Ryazantsev C.V. Comparison of Russian standards at treatment acute sinusitis with international program EPOS // Consilium medicum, 2008, no. 10, P. 87–90.

Kolosova N.G., Geppe N.A. Therapy by nebulizers in pediatric practice // PMJ, 2011, no. 8, P. 514–518.

Karpova E.P., Tulupov D.A. About possibility of nebulizer therapy in treatment of acute rhinosinusitis at children // Russian otorhinolaryngology, 2013, no. 65 (4), P. 160–163.

Kolesnikova M.B., Kilina A.V. Efficacy of use ethereal oils in prophylaxis of acute respiratory diseases at preschool children in organized offices // Bulletin on otorhinolaryngology, 2011, no. 5, P. 51–54.

Karpova E.P., Bojatova M.P. Rational methods of treatment ARVI at children // Pharmateka, 2008; 19: 89–92.

Karpova E.P., Sokolova M.B. Irrigational therapy of allergic rhinitis at children // Bulletin on otorhinolaryngology, 2007, no.5, P. 23–24.

Gramann J., Hippeli S., Dornisch K. et al. Antioxidant properties of essential oils, Arzneim. Forsch. // Drug. Res., 2000, vol. 50 (1), p. 135–139.

Petrushina A.D., Nikogosyan A.C., Kayb I.D. et al. Use inhalation with ethereal oils in complex therapy and for prophylaxis ARVI at children // Polyclinic, 2013; 4: 105–7.

Azova E.A. et al. Experience of inhalation use “Breathe” oil in prophylaxis and complex therapy of ARD at children. Reference book of pediatrician, 2012; 3: 55–61.

Boykova N.E., Garashenko T.I. Rationality of use ethereal oils in therapy of viral rhinosinusitis at children //Attending doctor. – 2018. – no. 9. – P. 36.

Baranov V.M. et al. Evaluation of microecological human status by the method chromato-mass spectrometry //New medical technology MH RF no. НЮ-40006. – 2009. – Т. 17.- P. 37.

Muhamadiev A.N., Fazlieva N.T., Muhamadiev N.Q. Study of the chemical composition of essential oil extracted from Pimpinella Anisum L. by GX-MS method // Scientific bulletin of Samarkand State University. - 2014. - No. 1 (83). – P. 83-87 (Uzb).

Published
2022-08-30
How to Cite
Karabaev H.E., Mamatova Sh.R., Muhamadiev N.K., Naimova B.K., & Berdimurodova F.P. (2022). Determination of microorganisms markers by the method GC-MS and efficacy evaluation of rhinosinusitis. Central Asian Journal of Medical and Natural Science, 3(4), 384-393. https://doi.org/10.17605/cajmns.v3i4.729
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Articles

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