Genetic Basis of Bladder Cancer: Predictive Roles of Interleukin Signaling Molecules

Hasanain Ali  Shubbar; Sarah Salih  Hasan; Hamsa Faisal  Najm

doi:10.17605/cajmns.v6i3.2825

Hasanain Ali Shubbar Jabir Ibn Hayyan University for Medical and Pharmaceutical Sciences, Faculty of Medicine.
Sarah Salih Hasan Jabir Ibn Hayyan University for Medical and Pharmaceutical Sciences, Faculty of Medicine.
Hamsa Faisal Najm College of Dentistry, Al-Bayan University

DOI: https://doi.org/10.17605/cajmns.v6i3.2825

Keywords: Bladder cancer, IL-6, rs1800795 polymorphism, Tetra-ARMS PCR, genetic susceptibility, inflammation, Iraqi population, genotypic analysis.

Abstract

Despite the fact that bladder cancer is a major public health problem in Iraq, the genetic elements that contribute to its development are still not fully understood. Through the use of a case-control approach, the purpose of this investigation is to determine whether or not the IL-6 rs1800795 (-174 G/C) polymorphism is associated with an increased risk of bladder cancer in the Iraqi population. Techniques. There were a total of 148 individuals in the research, including 78 cases and 70 controls. The participants were separated into two groups. Patients who had previously been diagnosed with bladder cancer by histological examination made up the case group. In cases, the GC genotype and the G allele were found to be significantly more prevalent than in controls (odds ratio = 2.22, p = 0.004). On the other hand, the GC and C alleles were found to be considerably more prevalent in cases. The outcomes. At the case group level, there were no statistically significant differences between the two groups in terms of the probability of developing bladder cancer (p 0.05). There was a statistically significant difference in the frequency of the GG genotype between the patients and the controls in the control group (P = $0.04). A further finding was that there was no statistically significant difference in the CC genotype between the patients and the controls (p = 0.001). I will conclude. Taking into consideration these findings, it appears that there may be a connection between the gastrointestinal genotype of the miR-146a gene and an elevated risk of lung cancer in Iraq. Given that both the miR146a SNP Rs2910164 and the miR 155 SNP rS767649 are risk factors for non-small cell lung cancer, it is important to emphasize the significance that genetic variables play in the genesis of lung cancer.

References

R. S. N. Al-Saeagh and A. H. Hassan, “Genomic DNA extraction from mice tissues by using two methods (favor prep tissue genomic DNA extraction kit and rapid method),” Biochem. Cell. Arch., vol. 19, no. 1, 2019.

M. H. Alyasiri and S. A. A. Atiyah, “Identify the urinary bladder cancer patterns in Nasiriyah city/Thi-Qar Province–Iraq,” Univ. Thi-Qar J. Sci., vol. 10, no. 2, pp. 87–91, 2023.

N. K. Dezfuli, I. M. Adcock, S. D. Alipoor, S. Seyfi, B. Salimi, M. M. Golchin, and E. Mortaz, “The miR‐146a SNP rs2910164 and miR‐155 SNP rs767649 are risk factors for non‐small cell lung cancer in the Iranian population,” Can. Respir. J., 2020.

Y. Jia et al., “MicroRNA-146a rs2910164 polymorphism is associated with susceptibility to non-small cell lung cancer in the Chinese population,” Med. Oncol., vol. 31, no. 194, 2014. [Online]. Available: https://doi.org/10.1007/s12032-014-0194-2

N. S. Omar and R. T. Ali, “Dosimetric analysis with intensity-modulated radiation therapy for central nervous system irradiation in patients with brain cancer compared with three-dimensional conformal radiation therapy treatment,” Polytech. J., vol. 9, no. 2, p. 9, 2019.

N. S. Omar, F. K. Ahmed, D. S. Saleem, L. J. A. Shah, and M. A. Abbas, “Influence of maternal body mass index on fetal ultrasound biometry,” Cihan Univ.-Erbil Sci. J., vol. 8, no. 2, pp. 93–98, 2024.

Y. G. Ren, X. M. Zhou, Z. G. Cui, and G. Hou, “Effects of common polymorphisms in miR-146a and miR-196a2 on lung cancer susceptibility: A meta-analysis,” J. Thorac. Dis., vol. 8, no. 6, pp. 1297–1305, 2016.

J. Wang, Q. Wang, H. Liu, et al., “The association of miR-146a rs2910164 and miR-196a2 rs11614913 polymorphisms with cancer risk: A meta-analysis of 32 studies,” Mutagenesis, vol. 27, no. 6, pp. 779–788, 2012.

I. I. Wistuba and A. F. Gazdar, “Lung cancer preneoplasia,” Annu. Rev. Pathol., vol. 1, pp. 331–348, 2006.

Z. Yin, Z. Cui, Y. Ren, L. Xia, H. Li, and B. Zhou, “MiR-146a polymorphism correlates with lung cancer risk in Chinese nonsmoking females,” Oncotarget, vol. 8, no. 2, pp. 2275–2283, 2017.

J. Zhang, W. Shao, Z. Zhang, and D. Liu, “The rate of miR-146a rs2910164 mutations in patients with lung cancer: A meta-analytic review,” Transl. Cancer Res., vol. 9, no. 11, pp. 6792–6800, 2020.

H. Chen and M. Xu, “Genetic variations of microRNAs and cancer susceptibility,” Oncotarget, vol. 9, no. 5, pp. 5012–5025, 2018.

S. H. Lee and J. Kim, “Emerging roles of microRNA mutations in cancer diagnostics,” Cancer Res., vol. 78, no. 13, pp. 3470–3476, 2018.

X. Li, Y. Chen, and Z. Zhang, “miRNA-related polymorphisms and cancer risk: A systematic review,” Clin. Genet., vol. 95, no. 5, pp. 569–578, 2019.

R. Zhao and K. Huang, “Functional analysis of miR-146a in cancer development,” Mol. Cancer Res., vol. 17, no. 7, pp. 1441–1450, 2019.