Newcastle Disease Hemato-immune Reaction Following Immunization

Batool Kadhum  Meteab; Dhiaa Jaber  Hamzah

doi:10.17605/cajmns.v6i2.2723

Batool Kadhum Meteab University of Al-Qadisiyah, Veterinary Collage, Pathology and Poultry Diseases Department, Iraq
Dhiaa Jaber Hamzah University of Al-Qadisiyah, Veterinary Collage, Pathology and Poultry Diseases Department, Iraq

DOI: https://doi.org/10.17605/cajmns.v6i2.2723

Keywords: ND, Vaccine Route, Antibodies, WBC Count, H/L Ratio

Abstract

One hundred unsexed Ross broiler chicks, one day old, were used in this experiment divided four groups as a control group, the first group (G1) was not immunized. The Newcastle Lasota strain was sprayed into the second group (G2) was revaccinated with drinking water with a second and third vaccine, third group (G3) was inoculated with the inactivated oil adjuvant vaccine via subcutaneous injection on the tenth day of the birds' age precede eye instillation attenuated live vaccine it is reinforced with live vaccine attenuated via drinking water, fourth group (G4) was administered the vaccine via eye instillation as a first vaccine then it was vaccinated with a second and a third with a live attenuated vaccine using the drinking water method. Newcastle antibody levels were determined using a hem agglutination inhibition (HI) test and an ELISA test for chick blood on 7, 14, and 28 days from all groups at the probability level (P 0.05), Hemoglobin inhibitory antibodies to the Newcastle antigen were significantly higher in the vaccinated groups (G2, G3, and G4) than control group (G1). When the vaccinated groups (G2, G3, and G4) were compared to the control group, the Newcastle antibody standard as determined by the ELISA test increased significantly (P 0.05). In terms of antibody levels as assessed by the agglutination inhibitor and ELISA tests, G3 did better than the other groups, followed by G4. White blood cell counts (WBC) (cells/mm3) and the ratio of Heterophils/Lymphocytes (H/L) were measured at 7, 14, and 28 days. When the vaccinated groups were compared to the negative control group, the rate of WBC increased significantly (P 0.05) and decrease the ratio of heterophils to lymphocytes implies stress as substantial (P 0.05) which reflected positively on the birds' health. G3 was significantly superior of the amount of white blood cells at value 28.1 2 ± 0.1 2 , 32.11 ± 0.16 on 14, 28 days of age , as well as a considerable drop in the percentage of heterophil cells H/L. especially on 14, 28 days from age at value 0.21 ± 0.006 , 0.18 ± 0.007 respectively.

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