Molecular Detection of Cytomegalovirus and Bacterial Species Causing Urinary Tract Infections in Hemodialysis Patients

Ibrahim Mohammed Saeed Hussein; Jalank  Hameed Mahmoud; Sazan  Seddiq Karem

doi:10.17605/cajmns.v6i1.2710

Ibrahim Mohammed Saeed Hussein Northern Technical University, Medical Technology Institute, Kirkuk
Jalank Hameed Mahmoud Northern Technical University, Medical Technology Institute, Kirkuk
Sazan Seddiq Karem Northern Technical University, Medical Technology Institute, Kirkuk

DOI: https://doi.org/10.17605/cajmns.v6i1.2710

Keywords: Cytomegalovirus, Kidney failure, Real-time PCR, UTI

Abstract

This study included 100 kidney failure patients. Five ml of blood was collected from each patient, the plasma was separated, and then the plasma samples were stored until DNA extraction was performed. Molecular detection of Cytomegalovirus (CMV) was done by polymerase chain reaction (PCR). Urine samples were also obtained for bacterial species. The results of the PCR technique revealed that 8 patients were infected with the CMV at a rate of 8 %, with 5 infections in males and 3 infections in females, with a rate of 8.9% and 6.8% respectively. Regarding the age group, the highest incidence of the virus was in 21-30 age group in males and the 41-50 age group in females. The results of bacterial detection show that E. coli cause the highest rate of urinary tract infections in hemodialysis patients infected with the CMV at 100%, followed by Klebsiella pneumonia at 60%. We conclude that patients receiving end-stage renal dialysis may be more susceptible to CMV replication while they get chronic hemodialysis.

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