Study of the Sensitivity Patterns of Some Antibiotics to Pseudomonas Aeruginosa Isolated From Patients with Wounds in Nasiriyah City
Keywords:
Antimicrobial resistance, Wound infection, Pseudomonas aeruginosa
Abstract
Pseudomonas aeruginosa is a Gram-negative pathogen known for causing infections in hospitalized patients, particularly due to its multidrug resistance mechanisms, which significantly increase morbidity and mortality. The growing prevalence of multidrug-resistant strains has severely limited treatment options, highlighting the urgent need for novel therapeutic approaches. This study aimed to evaluate the antibiotic susceptibility of P. aeruginosa isolates from wound infections in patients at Hussain Teaching Hospital, Nasiriyah, between February and September 2024. Out of 100 wound samples, 14 P. aeruginosa isolates were identified using biochemical, morphological, and culture analyses. Antibiotic susceptibility testing revealed that most isolates showed high resistance to trimethoprim/sulfamethoxazole and amoxicillin, while imipenem was the most effective antimicrobial agent. These findings underscore the importance of continuous monitoring of antibiotic resistance patterns and the need for alternative treatment strategies to combat drug-resistant infections.
References
[1] M. T. Madigan, J. M. Martinko, and Brock, Biology of Microorganisms, 11th ed. New Jersey, USA: Pearson Prentice Hall, 2006.
[2] J. Engel, "Pseudomonas aeruginosa Internalization by Non-Phagocytic Cells," Am. J. Med. Microbiol. Immunol., vol. 5, pp. 343-368, 2007.
[3] M. Mahboobi, F. Shahcheraghi, and M. M. Feizabadi, "Bactericidal Effects of Essential Oils from Clove, Lavender, and Geranium on Multidrug-Resistant Isolates from Pseudomonas aeruginosa," Iran. J. Biotechno., vol. 4, no. 2, pp. 137-140, 2006.
[4] J. F. Parsons, B. T. Greenhagen, K. Shi, K. Calabrese, H. Robinson, and J. E. Ladner, "Structural and Functional Analysis of the Pyocyanin Biosynthetic Protein PhzM from Pseudomonas aeruginosa," Biochemistry, vol. 469, no. 7, pp. 1821-1828, 2007.
[5] E. Kipnis and T. Sawa, "Targeting Mechanisms of Pseudomonas aeruginosa Pathogenesis," Med. Mal. Infect., vol. 36, pp. 78-91, 2006.
[6] M. H. Wilcox, T. G. Winstanley, and R. C. Spencer, "Epidemiology of Drug Resistance: Implications for a Post-Antimicrobial Era," J. Sci., vol. 257, pp. 1050-1055, 1994.
[7] T. Bjarnsholt, M. Kirketerp-Møller, P. Ø. Jensen, K. G. Madsen, and R. Phipps, "Why Chronic Wounds Will Not Heal: A Novel Hypothesis," Wound Repair Regen., vol. 16, no. 1, pp. 2-10, 2008.
[8] M. Kirketerp-Møller, P. Ø. Jensen, M. Fazli, K. G. Madsen, and J. T. Pedersen, "Distribution, Organization, and Ecology of Bacteria in Chronic Wounds," J. Clin. Microbiol., vol. 46, no. 8, pp. 2717-2722, 2008.
[9] R. G. Sibbald, H. Orsted, G. S. Schultz, P. Coutts, and D. Keast, "Preparing the Wound Bed: Focus on Infection and Inflammation," Ostomy Wound Manage., vol. 49, no. 11, 2003.
[10] T. R. Thomsen, M. S. Aasholm, V. B. Rudkjøbing, A. M. Saunders, T. Bjarnsholt, and M. Givskov, "The Bacteriology of Chronic Venous Leg Ulcers Examined by Culture-Independent Molecular Methods," Wound Repair Regen., vol. 18, no. 1, pp. 38-49, 2010.
[11] H. P. Schweizer, "Efflux as a Mechanism of Resistance to Antimicrobials in Pseudomonas aeruginosa and Related Bacteria," Genet. Mol. Res., vol. 2, pp. 48-62, 2003.
[12] H. Haleem, J. K. Tarrad, and I. A. Banyan, "Isolation of Pseudomonas aeruginosa from Clinical Cases and Environmental Samples, and Analysis of its Antibiotic Resistant Spectrum at Hilla Teaching Hospital," Med. J. Babylon, vol. 8, no. 4, pp. 45-52, 2011.
[13] S. Mukerjee, S. Chaki, S. Barman, S. Das, H. Koley, and G. Dasidar, "Effective Elimination of Drug Resistance Genes in Pseudomonas aeruginosa by Antipsychotic Agents Thioridazine," Curr. Res. Bacteriol., vol. 5, no. 1, pp. 36-41, 2012.
[14] B. Kleyn, Microbiology Experiments: A Health Science Perspective, 4th ed. New York, USA: McGraw-Hill, 2003.
[15] I. Nakasone, T. Kinjo, N. Yamane, K. Kisanuki, and C. M. Shiohira, "Laboratory-Based Evaluation of the Colorimetric VITEK 2 Compact System for Species Identification and the Advanced Expert System for Detection of Antimicrobial Resistances," Diagn. Microbiol. Infect. Dis., vol. 58, pp. 191-198, 2007.
[16] M. Kasse, B. Baars, S. Friedrich, F. Szabados, and S. G. Gatermann, "Performance of MicroScan Walkaway and Vitek 2 for Detection of Oxacillin Resistance in a Set of Methicillin-Resistant Staphylococcus aureus Isolates with Diverse Genetic Backgrounds," J. Clin. Microbiol., vol. 47, no. 8, pp. 2623-2625, 2009.
[17] P. A. Wayne, "Performance Standard for Antimicrobial Susceptibility Testing," Clin. Lab. Stand. Inst., 15th ed., CLSI/NCCLS M100-S15, 2005.
[18] A. K. Kheder, "Studies on Antibiotic Resistance by Plasmids of Pseudomonas aeruginosa," Ph.D. dissertation, Science Education College, Salahaddin University-Erbil, Kurdistan, Iraq, 2002.
[19] S. S. H. Salih, "Some Bacteriological and Molecular Genetic Studies of Pseudomonas aeruginosa Isolated from Different Environments," M.Sc. thesis, Higher Academy of Human and Scientific Studies, Iraq, 2008.
[20] A. Othman, "Antiplasmid (Curing) Effect of Alcoholic Extract of Rosmarinus Officinalis on Resistant Isolate of Pseudomonas aeruginosa," M.Sc. thesis, Medical Microbiology College of Medicine, Hawler Medical University, 2011.
[21] C. Langelotza, A. Mueller-Raua, S. Terziyskia, B. Raua, P. A. Gastmeierc, and J. Geffersc, "Gender-Specific Differences in Surgical Site Infections: An Analysis of 438,050 Surgical Procedures from the German National Nosocomial Infections Surveillance System," Viszeralmedizin, vol. 30, pp. 114-117, 2014.
[22] C. Henwood, D. Livermore, D. James, and M. Waner, "Antimicrobial Susceptibility of Pseudomonas aeruginosa: Results of a UK Survey and Evaluation," Br. J. Antimicrob. Chemother., vol. 47, pp. 789-799, 2001.
[23] A. T. Olayinka, B. O. Olayinka, and B. A. Onile, "Antibiotic Susceptibility and Plasmid Pattern of Pseudomonas aeruginosa from the Surgical Unit of a University Teaching Hospital in North Central Nigeria," J. Med. Med. Sci., vol. 1, no. 3, pp. 79-83, 2009.
[24] M. Pollack, G. L. Mandell, J. E. Bennett, and R. Dolin, "Pseudomonas aeruginosa," in Principles and Practice of Infectious Diseases, G. L. Mandell, J. E. Bennett, and R. Dolin, Eds. 5th ed., 2000.
[2] J. Engel, "Pseudomonas aeruginosa Internalization by Non-Phagocytic Cells," Am. J. Med. Microbiol. Immunol., vol. 5, pp. 343-368, 2007.
[3] M. Mahboobi, F. Shahcheraghi, and M. M. Feizabadi, "Bactericidal Effects of Essential Oils from Clove, Lavender, and Geranium on Multidrug-Resistant Isolates from Pseudomonas aeruginosa," Iran. J. Biotechno., vol. 4, no. 2, pp. 137-140, 2006.
[4] J. F. Parsons, B. T. Greenhagen, K. Shi, K. Calabrese, H. Robinson, and J. E. Ladner, "Structural and Functional Analysis of the Pyocyanin Biosynthetic Protein PhzM from Pseudomonas aeruginosa," Biochemistry, vol. 469, no. 7, pp. 1821-1828, 2007.
[5] E. Kipnis and T. Sawa, "Targeting Mechanisms of Pseudomonas aeruginosa Pathogenesis," Med. Mal. Infect., vol. 36, pp. 78-91, 2006.
[6] M. H. Wilcox, T. G. Winstanley, and R. C. Spencer, "Epidemiology of Drug Resistance: Implications for a Post-Antimicrobial Era," J. Sci., vol. 257, pp. 1050-1055, 1994.
[7] T. Bjarnsholt, M. Kirketerp-Møller, P. Ø. Jensen, K. G. Madsen, and R. Phipps, "Why Chronic Wounds Will Not Heal: A Novel Hypothesis," Wound Repair Regen., vol. 16, no. 1, pp. 2-10, 2008.
[8] M. Kirketerp-Møller, P. Ø. Jensen, M. Fazli, K. G. Madsen, and J. T. Pedersen, "Distribution, Organization, and Ecology of Bacteria in Chronic Wounds," J. Clin. Microbiol., vol. 46, no. 8, pp. 2717-2722, 2008.
[9] R. G. Sibbald, H. Orsted, G. S. Schultz, P. Coutts, and D. Keast, "Preparing the Wound Bed: Focus on Infection and Inflammation," Ostomy Wound Manage., vol. 49, no. 11, 2003.
[10] T. R. Thomsen, M. S. Aasholm, V. B. Rudkjøbing, A. M. Saunders, T. Bjarnsholt, and M. Givskov, "The Bacteriology of Chronic Venous Leg Ulcers Examined by Culture-Independent Molecular Methods," Wound Repair Regen., vol. 18, no. 1, pp. 38-49, 2010.
[11] H. P. Schweizer, "Efflux as a Mechanism of Resistance to Antimicrobials in Pseudomonas aeruginosa and Related Bacteria," Genet. Mol. Res., vol. 2, pp. 48-62, 2003.
[12] H. Haleem, J. K. Tarrad, and I. A. Banyan, "Isolation of Pseudomonas aeruginosa from Clinical Cases and Environmental Samples, and Analysis of its Antibiotic Resistant Spectrum at Hilla Teaching Hospital," Med. J. Babylon, vol. 8, no. 4, pp. 45-52, 2011.
[13] S. Mukerjee, S. Chaki, S. Barman, S. Das, H. Koley, and G. Dasidar, "Effective Elimination of Drug Resistance Genes in Pseudomonas aeruginosa by Antipsychotic Agents Thioridazine," Curr. Res. Bacteriol., vol. 5, no. 1, pp. 36-41, 2012.
[14] B. Kleyn, Microbiology Experiments: A Health Science Perspective, 4th ed. New York, USA: McGraw-Hill, 2003.
[15] I. Nakasone, T. Kinjo, N. Yamane, K. Kisanuki, and C. M. Shiohira, "Laboratory-Based Evaluation of the Colorimetric VITEK 2 Compact System for Species Identification and the Advanced Expert System for Detection of Antimicrobial Resistances," Diagn. Microbiol. Infect. Dis., vol. 58, pp. 191-198, 2007.
[16] M. Kasse, B. Baars, S. Friedrich, F. Szabados, and S. G. Gatermann, "Performance of MicroScan Walkaway and Vitek 2 for Detection of Oxacillin Resistance in a Set of Methicillin-Resistant Staphylococcus aureus Isolates with Diverse Genetic Backgrounds," J. Clin. Microbiol., vol. 47, no. 8, pp. 2623-2625, 2009.
[17] P. A. Wayne, "Performance Standard for Antimicrobial Susceptibility Testing," Clin. Lab. Stand. Inst., 15th ed., CLSI/NCCLS M100-S15, 2005.
[18] A. K. Kheder, "Studies on Antibiotic Resistance by Plasmids of Pseudomonas aeruginosa," Ph.D. dissertation, Science Education College, Salahaddin University-Erbil, Kurdistan, Iraq, 2002.
[19] S. S. H. Salih, "Some Bacteriological and Molecular Genetic Studies of Pseudomonas aeruginosa Isolated from Different Environments," M.Sc. thesis, Higher Academy of Human and Scientific Studies, Iraq, 2008.
[20] A. Othman, "Antiplasmid (Curing) Effect of Alcoholic Extract of Rosmarinus Officinalis on Resistant Isolate of Pseudomonas aeruginosa," M.Sc. thesis, Medical Microbiology College of Medicine, Hawler Medical University, 2011.
[21] C. Langelotza, A. Mueller-Raua, S. Terziyskia, B. Raua, P. A. Gastmeierc, and J. Geffersc, "Gender-Specific Differences in Surgical Site Infections: An Analysis of 438,050 Surgical Procedures from the German National Nosocomial Infections Surveillance System," Viszeralmedizin, vol. 30, pp. 114-117, 2014.
[22] C. Henwood, D. Livermore, D. James, and M. Waner, "Antimicrobial Susceptibility of Pseudomonas aeruginosa: Results of a UK Survey and Evaluation," Br. J. Antimicrob. Chemother., vol. 47, pp. 789-799, 2001.
[23] A. T. Olayinka, B. O. Olayinka, and B. A. Onile, "Antibiotic Susceptibility and Plasmid Pattern of Pseudomonas aeruginosa from the Surgical Unit of a University Teaching Hospital in North Central Nigeria," J. Med. Med. Sci., vol. 1, no. 3, pp. 79-83, 2009.
[24] M. Pollack, G. L. Mandell, J. E. Bennett, and R. Dolin, "Pseudomonas aeruginosa," in Principles and Practice of Infectious Diseases, G. L. Mandell, J. E. Bennett, and R. Dolin, Eds. 5th ed., 2000.
Published
2024-10-13
How to Cite
Lhwak, N. S. (2024). Study of the Sensitivity Patterns of Some Antibiotics to Pseudomonas Aeruginosa Isolated From Patients with Wounds in Nasiriyah City. Central Asian Journal of Medical and Natural Science, 5(4), 996-1001. Retrieved from https://cajmns.centralasianstudies.org/index.php/CAJMNS/article/view/2650
Section
Articles
This work is licensed under a Creative Commons Attribution 4.0 International License.
