The Synergistic Effect of Cordia Myxa and Bioemulsifier Extracted from Saccharomyces Cerevisiae Against Pathogenic Bacteria

Ali Y.A. Saleh

Ali Y.A. Saleh Department of Science, College of Basic Education, University of Al-Mustansiriah, Baghdad, Iraq

Keywords: Cordia Myxa, Saccharomyces Cerevisiae, Bioemulsifier

Abstract

Twenty-five pathogenic bacterial isolates were obtained from different isolation sources. Most of the isolates showed resistance to most antibiotics. Five isolates of baker's yeast of different origins were collected from local markets. Isolate Pa was selected as the most efficient isolate in producing the bioemulsifier, with an emulsifying efficiency of E24 = 81.8%. The method of applying the microtiter plate was adopted to test the inhibitory activity. The bioemulsion showed inhibitory activity against six selected isolates at MIC=80.82mg/ml. Both extracts from C. myxa contain phenols, flavonoids, and glycosides, while the water extract without the alcoholic extract lacks alkaloids and a small percentage of saponin. The water extract showed inhibitory activity against S. Typhi, Shigella sonnei, P. aeruginosa, S. aureus, and K. pneumoniae at (MIC) = 250mg/ml, And at MIC=62.5mg/ml towards E. coli. The alcoholic extract showed inhibitory activity against S. Typhi, Shigella sonnei, K. pneumoniae, and E. coli isolates at MIC=62.5mg/ml, While it showed inhibitory activity against P. aeruginosa and S. aureus isolates at MIC=125mg/ml. The Boardchequer method was adopted to study the synergistic relationship and determine the FIC. The study showed a synergistic relationship between the bioemulsifier and the alcoholic extract with a fractional inhibitory concentration (FIC) = 0.267 towards isolates of S. typhi, Shigella sonnei, K. pneumonia, and E. coli, While the FIC = 0.151 towards P. aeruginosa and S. aureus isolates.

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