Evaluation of Aloe Vera Ethanolic Extract and Medical Al-Athmad Eyeliner on Bacterial Species Causing Eye Inflammation

Nagham Naseif Jassim  Al Khazraji; Yassien Hussain Owaied  Al-Juboory; Rashid Hamid  Hassan

Nagham Naseif Jassim Al Khazraji Department of Biology, College of Education for Women, University of Tikrit, Iraq
Yassien Hussain Owaied Al-Juboory National University of Science and Technology, Dhi Qar, College of Dentistry
Rashid Hamid Hassan Department of Biology, College of Education, University of Samarra, Iraq

Keywords: Extracts, Antimony kohl, Aloe vera, bacterial inhibition, ophthalmitis

Abstract

The current study included the collection of (300) samples from (150) people from patients visiting Balad General Hospital and some private clinics in Balad District - Salah Al-Din Governorate, a sample was taken from both the right and left eyes using special dry and sterile cotton swabs that can be disposed of later, for the period ranging from November 2022 to January 2023, (235) positive samples for bacterial growth were obtained, constituting 78.3% of the total samples collected, positive growth specimens have been diagnosed depending on their morphological, farming and biochemical properties, the diagnosis of a number of them was confirmed using the Vitek2 system. The results showed obtaining (140) isolates out of a total of (235) positive samples for Gram stain, with a percentage of 59.6%, it included aureus staphylococcus by 27.65%, Staphylococcus epidermidis by 14.89%, Bacillus by 6.36%, Streptococcus viridans by 4.25% and Diphtheria by 6.38%, while the negative isolates of Gram stain were (95) isolates with a percentage of 40.4% of the total isolates and included Psedomonas auroginosa by 14.89%, klebsilla bacteria by 8.51%, E-Coli bacteria by 10.63% and Nisseria gonorrhoeae bacteria by 6.38%. The results also showed that the eyeliner used in the study (Al-Athmad) gave a modest inhibitory activity compared to the ethanolic alcoholic extract against the bacterial species under study, the highest average inhibitory diameter was (15) mm for N. gonorrhoeae and S. viridans at concentration (200) mg/ml.

References

S. M. Kadham, M. A. Mustafa, N. K. Abbass, and S. Karupusamy, "IoT and Artificial Intelligence–Based Fuzzy-Integral N-Transform for Sustainable Groundwater Management," Applied Geomatics, vol. 16, no. 1, pp. 1-8, 2024.

S. H. Ali, H. S. Armeet, M. A. Mustafa, and M. T. Ahmed, "Complete Blood Count for COVID-19 Patients Based on Age and Gender," in AIP Conference Proceedings, vol. 2394, no. 1, Nov. 2022.

O. M. Shakir, K. K. Abdulla, A. A. Mustafa, and M. A. Mustafa, "Investigation of the Presence of Parasites that Contaminate Some Fruits and Vegetables in the Samarra City in Iraq," Plant Arch, vol. 19, pp. 1184-1190, 2019.

R. A. Astley, H. M. Md, S. C. Phillip, T. Erin, J. W. N. Livingston, B. Eddy, J. H. Jonathan, and M. C. Michelle, "Ocular Bacterial Infections: A Ten-Year Survey and Review of Causative Organisms Based on the Oklahoma Experience," Microorganisms, vol. 11, no. 7, p. 1802, 2023.

S. M. Kadham, M. A. Mustafa, N. K. Abbass, and S. Karupusamy, "Comparison Between of Fuzzy Partial H-Transform and Fuzzy Partial Laplace Transform in X-Ray Images Processing of Acute Interstitial Pneumonia," International Journal of System Assurance Engineering and Management, pp. 1-9, 2023.

D. G. J. Larsson and F. F. Carl, "Antibiotic Resistance in the Environment," Nature Reviews Microbiology, vol. 20, no. 5, pp. 257–69, 2022. doi: 10.1038/s41579-021-00649-x.

M. A. Meri, M. D. Ibrahim, A. H. Al-Hakeem, and M. A. Mustafa, "Procalcitonin and NLR Measurements in COVID-19 Patients," Latin American Journal of Pharmacy, pp. 220-223, 2023.

E. Buksh, S. A. Naz, A. Zubair, K. Yasmeen, M. Shafique, N. Jabeen, and M. Hamayun, "A Widely Used Eye Cosmetic with Hazardous Biochemical Composition," Biosci Biotech Res Asia, vol. 17, no. 3, 2020.

D. Valluru, M. A. Mustafa, H. Y. Jasim, K. Srikanth, M. V. L. N. RajaRao, and P. S. S. Sreedhar, "An Efficient Classroom Teaching Learning Method Using Augmented Reality," in 2023 9th International Conference on Advanced Computing and Communication Systems (ICACCS), vol. 1, pp. 300-303, Mar. 2023.

J. P. Harley and L. M. Prescott, Laboratory Exercises in Microbiology, 5th ed. New York: McGraw-Hill Companies, Inc., 2002, pp. 292-293.

M. Khanal, S. Lamichhane, A. Bhattarai, B. L. Kayastha, and S. N. Labh, "Extract of Aloe Vera (Aloe Barbadensis Miller) Enhance the Growth, Protein Contents and Gastrosomatic. Index (GaSI) of Common Carp Cyprinus Capio," Journal of Nutrition and Metabolism, 2021.

R. Levesque, SPSS Programming and Data Management, 4th ed., Chicago, 2007, p. 522.

H. F. Lahij and S. A. L. Safaa, "Multidrug-Resistant Clinical K. Pneumoniae ST16, ST218, and ST283 and Emergence of Pandrug-Resistant KPC-Positive ST6434/K2 Lineage in Iraq," Brazilian Journal of Microbiology, 2023. doi: 10.1007/s42770-023-01205-w.

K. Kitagawa, K. Shigemura, F. Yamamichi, L. Alimsa-djono, D. Rahardjo, K. Kuntaman, and M. Fujisawa, "International Comparison of Causative Bacteria and Antimicrobial Susceptibilities of Urinary Tract Infections Between Kobe, Japan, and Surabaya, Indonesia," Japanese Journal of Infectious Diseases, vol. 71, no. 1, pp. 8-13, 2018.

S. A. H. Hassan, "Determine the Effectiveness of Some Plant Extracts Towards Some Types of Tachyria Isolated from the Eyes of Students Using Contact Lenses and Complements at the University of Tikrit," M.S. thesis, Faculty of Education for Pure Sciences, University of Tikrit, 2019.

M. D. Willcox, "Characterization of the Normal Microbiota of the Ocular Surface," Experimental Eye Research, vol. 117, pp. 99-105, 2013.

A. H. Yaseen, A. T. Khalaf, and M. A. Mustafa, "Lung Cancer Data Analysis for Finding Gene Expression," Afr. J. Biol. Sci., vol. 5, no. 3, pp. 119-130, 2023.

Z. F. Lu, C. Y. Hsu, N. K. Younis, M. A. Mustafa, E. A. Matveeva, Y. H. O. Al-Juboory, ... and M. N. Abdulraheem, "Exploring the Significance of Microbiota Metabolites in Rheumatoid Arthritis: Uncovering Their Contribution from Disease Development to Biomarker Potential," APMIS, 2024.

A. Hrycko, M-G. Pedro, C. Courtney, L-W. Rebecca, and E. Benjamin, "Severe Bacterial Infections in People Who Inject Drugs: The Role of Injection-Related Tissue Damage," Harm Reduction Journal, vol. 19, no. 1, pp. 1–13, 2022. doi: 10.1186/s12954-022-00624-6.

M. J. Saadh, F. R. B. Avecilla, M. A. Mustafa, A. Kumar, I. Kaur, Y. M. Alawayde, ... and Y. Elmasry, "The Promising Role of Doped h-BANDs for Solar Cells Application: A DFT Study," Journal of Photochemistry and Photobiology A: Chemistry, vol. 451, p. 115499, 2024.

Z. H. Mahmoud, Y. Ajaj, A. M. Hussein, H. N. K. Al-Salman, M. A. Mustafa, E. H. Kadhum, ... and E. Kianfar, "CdIn2Se4@Chitosan Heterojunction Nanocomposite with Ultrahigh Photocatalytic Activity Under Sunlight Driven Photodegradation of Organic Pollutants," International Journal of Biological Macromolecules, vol. 267, p. 131465, 2024.

M. J. Saadh, M. A. Mustafa, N. M. Hussein, P. Bansal, H. Kaur, M. H. S. Alubiady, ... and S. Margarian, "Investigating the Ability of BC2N Nanotube to Remove Eriochrome Blue Black from Wastewater: A Computational Approach," Inorganic Chemistry Communications, vol. 163, p. 112311, 2024.

P. Danish, Q. Ali, M. M. Hafeez, and A. Malik, "Antifungal and Antibacterial Activity of Aloe Vera L Plant Extract," Biological and Clinical Science Research Journal, 2020, vol. 1.

M. S. Junejo and P. A-A. Aziz, "Kohl (SURMA) on CT Scan–An Incidental Finding," Pakistan Armed Forces Medical Journal, vol. 72, no. 4, pp. 1492–93, 2022.

A. Amiri, H. A. Ehsan, and M. Abdolali, "Memorial Functional Foods: A New Concept from Bavi Tribe," Journal of Ethnic Foods, vol. 7, pp. 1–10, 2020.

O. M. Badeeb, S. A. Radwan, and H. W. Mohammed, "Kohl Al-Ethmed," Journal of King Abdulaziz University-Medical Sciences, vol. 15, no. 4, p. 5967, 2008.

A. A. Al-Kaff, T. Khalid, and El-Yazigi Adnan, "Kohl-the Traditional Eyeliner: Use and Analysis," Annals of Saudi Medicine, vol. 13, no. 1, pp. 26–30, 1993.

Z. A. Mahmood, I. Azhar, S. W. Ahmed, and P. Wexler, "Toxicology in Antiquity," 2019.

R. Gupta, "Formulation, Preliminary Evaluation and Antimicrobial Activity of a Herb Based Kohl," International Journal of Phytocosmetics and Natural Ingredients, vol. 3, no. 1, p. 5, 2016.

M. Jarosz, O. Magdalena, W. Gabriela, M. Katarzyna, and L. Tadeusz, "Antioxidant and Anti-Inflammatory Effects of Zinc. Zinc-Dependent NF-ΚB Signaling," Inflammopharmacology, vol. 25, pp. 11–24, 2017.

A. T. Abdulqader, A. M. Y. Al-Sammarie, and M. A. Mustafa, "A Comparative Environmental Study of Aqueous Extracts of Ginger and Grapes to Protect Hepatocytes in Albino Rabbits and a Comparison of Extracts in Preserving Awassi Lamb Meat from Oxidation," in IOP Conference Series: Earth and Environmental Science, vol. 1029, no. 1, p. 012001, May 2022.

R. Gupta, "Formulation, Preliminary Evaluation and Antimicrobial Activity of a Herb Based Kohl," International Journal of Phytocosmetics and Natural Ingredients, vol. 3, no. 1, p. 5, 2016.

C. Y. Hsu, M. A. Mustafa, A. Yadav, K. M. Batoo, M. Kaur, S. Hussain, ... and L. Nai, "N2 Reduction to NH3 on Surfaces of Co-Al18P18, Ni-Al21N21, Fe-B24N24, Mn-B27P27, Ti-C60 and Cu-Si72 Catalysts," Journal of Molecular Modeling, vol. 30, no. 3, pp. 1-11, 2024.

J. Hus, A. T. Gerstenblith, H. S. J. Garg, and J. F. Vander, "Conjunctival Flora Antibiotic Resistance Patterns After Serial Intravitreal Injections Without Postinjection Topical Antibiotics," American Journal of Ophthalmology, vol. 157, no. 3, pp. 514-518, 2014.

M. A. Mustafa, S. M. Kadham, N. K. Abbass, S. Karupusamy, H. Y. Jasim, B. A. Alreda, ... and M. T. Ahmed, "A Novel Fuzzy M-Transform Technique for Sustainable Ground Water Level Prediction," Applied Geomatics, vol. 16, no. 1, pp. 9-15, 2024.

M. A. Mustafa, S. Raja, L. A. A. Asadi, N. H. Jamadon, N. Rajeswari, and A. P. Kumar, "A Decision-Making Carbon Reinforced Material Selection Model for Composite Polymers in Pipeline Applications," Advances in Polymer Technology, vol. 2023, no. 1, p. 6344193, 2023.