Molecular Investigation of Carbapenems KPC and NDM Genes Among Klebsiella Pneumoniae Strains Isolated from Various Clinical Samples in Al-Basrah Province, Iraq

Duha Hassan  Abdul-Wahab; Saad Shakir Mahdi  Al-Amara; Talib Abdulmaged  Alboslemy

Duha Hassan Abdul-Wahab M.Sc. Student, Department of Biology, College of Science, University of Basrah, Iraq
Saad Shakir Mahdi Al-Amara Department of Pathological analyses, College of Science, University of Basrah, Iraq
Talib Abdulmaged Alboslemy Department of Biology, College of Science, University of Basrah, Iraq

Keywords: Klebsiella pneumoniae, carbapenemase, blaKPC and blaNDM genes

Abstract

Carbapenem is the final defense against Gram-negative ESBLs producing antibiotics, with resistance to Enterobacteriaceae, particularly Carbapenem-resistant Klebsiella pneumoniae (CR-KP), posing a significant public health threat. The samples for the current investigation were from three hospitals in the Iraqi, region of Al-Basrah. The n = 26 K. pneumoniae isolates were previously collected by the master's study between October 2022 and December 2022. The K. pneumoniae isolates were reactivated and reidentified using a PCR technique using the diagnostic gene 16S rDNA. The isolates were then grown on MacConkey agar, K.pneumoniae chromogenic media and confirm identified by PCR technique by using specific primer for K.pneumoniae had a molecular weight of approximately 130 bp. The results showed that all n = 26 isolates are (100%) K. pneumoniae.Modified Hodge Test (MHT) was performed on all n=26 K. pneumoniae isolates.The results in the current study showed that out of n=26 K.pneumoniae isolates the 19(73%) isolates gave positive results for production of carbapenemase.While the 7(26.923%) isolates were showed negative results for produced carbapenemase. Carbapenemase genes was detected by using two specific primers bla_KPC and bla_NDM genes. The amplified genes' bands were characterized approximately at (480 bp) and (621) respectively, compared to the stander molecular DNA ladder at (2000 bp), results were shown the all n=26 K. pneumoniae isolates had gave negative results for the bla_KPCgene. On the other hand, only 17(65.384%) K.pneumoniae isolates were gave positive results for detection of bla_NDM gene. While 9(34.615%) K.pneumoniae isolates were gave negative results for detection the bla_NDM gene.

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