Improved Histological Effect of Salmonella Typhimurium Ghost Vaccine Used in the Treatment of Colon Cancer Induced in Male Albino Rats
Keywords:
bacterial ghost, colorectal cancer, histo-immune stimulation, rats, vaccination
Abstract
Colorectal cancer continues among most serious life-threatening tumors worldwide. Recently, as a result of the progress made in microbial science and its relationship to cancer, and the efforts made to eliminate this dangerous disease without affecting normal cells, the current study aimed to shed light on Bacteria-mediated cancer therapy as a promising therapeutic tool with less side effects and due to its high immune stimulation effect. Colon cancer was induced in male albino rats using Azoxymethane (AOM) at 15mg/Kg bw, the curative effect (post-treatment) of Salmonella typhimurium ghosts (STGs), which was prepared using chemical concentrations selected in a special protocol, was studied by inoculated the animal subcutaneously with a 100 μl/rat in single dose with equal volumes (1:1) of STGs +Freund's adjuvant. We vaccinated the animal with (STGs) in 3 cycles. After 42 days of the whole vaccination, the animals were sacrificed, the colorectal tissues were removed and preserved in a diluted formalin solution until performing grossly and histological examinations. The result revealed that histolpathological changes in induced colorectal cancer includes damage and alterations in mucosal crypts architecture, increase spaces between crypts, dysplastic changes in crypts epithelium. In vaccine treated animal showing severe histological alterations, infiltration of inflammatory cells and significant hyperplasia in lymphoid tissue, comparing with the control group. In conclusion, vaccination by STGs vaccine using S. typhimurium will enhance the immune response, which is a promising medication for the treatment of colon cancer.
References
F. Bray, J. Ferlay, I. Soerjomataram, and ..., “Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries,” CA: a cancer journal …, 2018, doi: 10.3322/CAAC.21492.
F. Rommasi, “Bacterial-based methods for cancer treatment: What we know and where we are,” Oncol Ther, 2022, doi: 10.1007/s40487-021-00177-x.
D. Wang, X. Wei, D. V Kalvakolanu, B. Guo, and ..., “Perspectives on Oncolytic Salmonella in Cancer Immunotherapy—A Promising Strategy,” Frontiers in …, 2021, doi: 10.3389/fimmu.2021.615930.
S. Riedel, S. Morse, T. Mietzner, S. Miller, and ..., Adelberg’s medical microbiology. … York: McGrawHill Education/Medical, 2019.
M. S. Sadiq and R. M. Othman, Phylogenetic tree constructed of Salmonella enterica subspecies enterica isolated from animals and humans in Basrah and Baghdad governorates, Iraq. iasj.net, 2022. [Online]. Available: https://www.iasj.net/iasj/download/23fdebe6f049a677
F. H. Abdullah and N. M. Al-Gburi, “Risk factors assessment and antimicrobial resistance of Salmonella isolates from apparently healthy and diarrheal dogs in Baghdad, Iraq,” Iraqi Journal of Veterinary Sciences, 2024, [Online]. Available: https://www.iasj.net/iasj/download/f668f85650b8e4f5
L. A. Razzak, N. A. Hisham, M. M. A. Darwish, and ..., “Efficacy of vaccine from whole killed Vibrio alginolyticus cells on the immune response of white shrimp (Litopenaeus vannamei),” Iraqi Journal of Veterinary …, 2024, [Online]. Available: https://www.iasj.net/iasj/download/a0424b3c32fcfc93
D. Ling et al., “Cancer cell membrane-coated bacterial ghosts for highly efficient paclitaxel delivery against metastatic lung cancer,” … Pharmaceutica Sinica B, 2024, [Online]. Available: https://www.sciencedirect.com/science/article/pii/S2211383523003167
R. H. Ali, M. E. Ali, and R. Samir, “Production and Characterization of Bacterial Ghost Vaccine against Neisseria meningitidis,” Vaccines (Basel), 2022, [Online]. Available: https://www.mdpi.com/2076-393X/11/1/37
A. M. Batah and T. A. Ahmad, “The development of ghost vaccines trials,” Expert Rev Vaccines, 2020, doi: 10.1080/14760584.2020.1777862.
S. Rabea, A. S. Yassin, A. F. Mohammed, and ..., “Immunological characterization of the chemically prepared ghosts of Salmonella Typhimurium as a vaccine candidate,” BMC Veterinary …, 2022, doi: 10.1186/s12917-021-03112-4.
A. Muhammad, J. Champeimont, U. B. Mayr, and ..., “Bacterial ghosts as carriers of protein subunit and DNA-encoded antigens for vaccine applications,” Expert review of …, 2012, doi: 10.1586/erv.11.149.
N. Vinod et al., “A Salmonella typhimurium ghost vaccine induces cytokine expression in vitro and immune responses in vivo and protects rats against homologous and …,” PLoS …, 2017, [Online]. Available: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0185488
A. A. Amro, A. J. Neama, A. Hussein, E. A. Hashish, and ..., “Evaluation the Surface Antigen of the Salmonella typhimurium ATCC 14028 Ghosts Prepared by ‘SLRP,’” The Scientific World …, 2014, [Online]. Available: https://www.hindawi.com/journals/tswj/2014/840863/abs/
J. C. Garber, “Guide for the Care and Use of Laboratory Animals: National Research Council,” The National Academies Press, Washington, DC. https …, 2011.
A. A. Jabbar, I. A. A. Ibrahim, F. O. Abdullah, K. F. Aziz, and ..., “Chemopreventive Effects of Onosma mutabilis against Azoxymethane-Induced Colon Cancer in Rats via Amendment of Bax/Bcl-2 and NF-κB Signaling …,” Current Issues in …, 2023, [Online]. Available: https://www.mdpi.com/1467-3045/45/2/57
E. Nascimento-Gonçalves, B. A. L. Mendes, and ..., “Animal models of colorectal cancer: from spontaneous to genetically engineered models and their applications,” Veterinary …, 2021, [Online]. Available: https://www.mdpi.com/2306-7381/8/4/59
A. I. Thaker, A. Shaker, M. S. Rao, and M. A. Ciorba, “Modeling colitis-associated cancer with azoxymethane (AOM) and dextran sulfate sodium (DSS),” JoVE (Journal of Visualized …, 2012, [Online]. Available: https://www.jove.com/t/4100/modeling-colitis-associated-cancer-with-azoxymethane-aom-dextran
R. P. Bird, “Observation and quantification of aberrant crypts in the murine colon treated with a colon carcinogen: preliminary findings,” Cancer Lett, 1987, [Online]. Available: https://www.sciencedirect.com/science/article/pii/0304383587901571
J. D. Bancroft and M. Gamble, Theory and practice of histological techniques. books.google.com, 2008. [Online]. Available: https://books.google.com/books?hl=en&lr=&id=Dhn2KispfdQC&oi=fnd&pg=PR13&dq=theory+and+practice+of+histological+techniques+7+th&ots=JCjDcCUBD6&sig=-j7qWpcH8WtJauoPNbm4EGQaOl8
W. Yu, J. Zhang, Z. Chen, S. Wang, C. Ruan, and ..., “Inhibitory effect of a microecological preparation on azoxymethane/dextran sodium sulfate-induced inflammatory colorectal cancer in mice,” Frontiers in …, 2020, doi: 10.3389/fonc.2020.562189.
V. F. Machado, M. R. Feitosa, J. J. R. Rocha, and ..., “A review of experimental models in colorectal carcinogenesis,” Journal of Coloproctology …, 2016, [Online]. Available: https://www.scielo.br/j/jcol/a/hy9zQ7fVT5fV7PpmpHDzgHG/
K. Vdoviaková, E. Petrovová, M. Maloveská, and ..., “Surgical anatomy of the gastrointestinal tract and its vasculature in the laboratory rat,” … research and practice, 2016, [Online]. Available: https://www.hindawi.com/journals/grp/2016/2632368/abs/
S. S. Arango-Varela, I. Luzardo-Ocampo, and ..., “Andean berry (Vaccinium meridionale Swartz) juice in combination with Aspirin modulated anti-inflammatory markers on LPS-stimulated RAW 264.7 macrophages,” Food research …, 2020, [Online]. Available: https://www.sciencedirect.com/science/article/pii/S0963996920305664
M. M. Derry, K. Raina, R. Agarwal, and C. Agarwal, “Characterization of azoxymethane-induced colon tumor metastasis to lung in a mouse model relevant to human sporadic colorectal cancer and evaluation of grape …,” Experimental and Toxicologic …, 2014, [Online]. Available: https://www.sciencedirect.com/science/article/pii/S0940299314000293
F. Gossé, S. Guyot, S. Roussi, A. Lobstein, and ..., “Chemopreventive properties of apple procyanidins on human colon cancer-derived metastatic SW620 cells and in a rat model of colon carcinogenesis,” …, 2005, [Online]. Available: https://academic.oup.com/carcin/article-abstract/26/7/1291/2390898
W. Li and C. B. Li, “Lack of inhibitory effects of lactic acid bacteria on 1, 2-dimethylhydrazine-induced colon tumors in rats,” World J Gastroenterol, 2003, [Online]. Available: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4656522/
R. Suzuki, H. Kohno, S. Sugie, H. Nakagama, and ..., “Strain differences in the susceptibility to azoxymethane and dextran sodium sulfate-induced colon carcinogenesis in mice,” …, 2005, [Online]. Available: https://academic.oup.com/carcin/article-abstract/27/1/162/2390964
R. L. Johnson and J. C. Fleet, “Animal models of colorectal cancer,” Cancer and Metastasis Reviews, 2013, doi: 10.1007/s10555-012-9404-6.
M. Al-Obaybidi, Study the Effect of Lactobacillus acidophilus Local Isolate and its Components as Anticancer in vitro and in vivo. PhD thesis. Genetic Engineering …, 2013.
D. Kocijancic, S. Leschner, S. Felgner, R. M. Komoll, and ..., “Therapeutic benefit of Salmonella attributed to LPS and TNF-α is exhaustible and dictated by tumor susceptibility,” Oncotarget, 2017, [Online]. Available: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5482671/
C. H. Lee, C. L. Wu, and A. L. Shiau, “Toll-like Receptor 4 Mediates an Antitumor Host Response Induced by Salmonella choleraesuis,” Clinical Cancer Research, 2008, [Online]. Available: https://aacrjournals.org/clincancerres/article-abstract/14/6/1905/196343
S. Grille, M. Moreno, T. Bascuas, J. M. Marqués, and ..., “Salmonella enterica serovar Typhimurium immunotherapy for B‐cell lymphoma induces broad anti‐tumour immunity with therapeutic effect,” …, 2014, doi: 10.1111/imm.12320.
Y. Barak, F. Schreiber, S. H. Thorne, C. H. Contag, and ..., “Role of nitric oxide in Salmonella typhimurium-mediated cancer cell killing,” BMC Cancer, 2010, doi: 10.1186/1471-2407-10-146.
S. Kaimala, Y. A. Mohamed, N. Nader, J. Issac, and ..., “Salmonella-mediated tumor regression involves targeting of tumor myeloid suppressor cells causing a shift to M1-like phenotype and reduction in suppressive …,” Cancer Immunology …, 2014, doi: 10.1007/s00262-014-1543-x.
C. H. Lee, J. L. Hsieh, C. L. Wu, H. C. Hsu, and ..., “B cells are required for tumor-targeting Salmonella in host,” Applied microbiology and …, 2011, doi: 10.1007/s00253-011-3386-0.
C. H. Lee, J. L. Hsieh, C. L. Wu, P. Y. Hsu, and A. L. Shiau, “T cell augments the antitumor activity of tumor-targeting Salmonella,” Applied microbiology and …, 2011, doi: 10.1007/s00253-011-3180-z.
I. J. Lafta, “Crude Anthrax Protective Antigen Enhances Immunity For Salmonella Typhimurium in Mice,” J Fac Med Baghdad, 2017, [Online]. Available: https://www.iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/133
F. Rommasi, “Bacterial-based methods for cancer treatment: What we know and where we are,” Oncol Ther, 2022, doi: 10.1007/s40487-021-00177-x.
D. Wang, X. Wei, D. V Kalvakolanu, B. Guo, and ..., “Perspectives on Oncolytic Salmonella in Cancer Immunotherapy—A Promising Strategy,” Frontiers in …, 2021, doi: 10.3389/fimmu.2021.615930.
S. Riedel, S. Morse, T. Mietzner, S. Miller, and ..., Adelberg’s medical microbiology. … York: McGrawHill Education/Medical, 2019.
M. S. Sadiq and R. M. Othman, Phylogenetic tree constructed of Salmonella enterica subspecies enterica isolated from animals and humans in Basrah and Baghdad governorates, Iraq. iasj.net, 2022. [Online]. Available: https://www.iasj.net/iasj/download/23fdebe6f049a677
F. H. Abdullah and N. M. Al-Gburi, “Risk factors assessment and antimicrobial resistance of Salmonella isolates from apparently healthy and diarrheal dogs in Baghdad, Iraq,” Iraqi Journal of Veterinary Sciences, 2024, [Online]. Available: https://www.iasj.net/iasj/download/f668f85650b8e4f5
L. A. Razzak, N. A. Hisham, M. M. A. Darwish, and ..., “Efficacy of vaccine from whole killed Vibrio alginolyticus cells on the immune response of white shrimp (Litopenaeus vannamei),” Iraqi Journal of Veterinary …, 2024, [Online]. Available: https://www.iasj.net/iasj/download/a0424b3c32fcfc93
D. Ling et al., “Cancer cell membrane-coated bacterial ghosts for highly efficient paclitaxel delivery against metastatic lung cancer,” … Pharmaceutica Sinica B, 2024, [Online]. Available: https://www.sciencedirect.com/science/article/pii/S2211383523003167
R. H. Ali, M. E. Ali, and R. Samir, “Production and Characterization of Bacterial Ghost Vaccine against Neisseria meningitidis,” Vaccines (Basel), 2022, [Online]. Available: https://www.mdpi.com/2076-393X/11/1/37
A. M. Batah and T. A. Ahmad, “The development of ghost vaccines trials,” Expert Rev Vaccines, 2020, doi: 10.1080/14760584.2020.1777862.
S. Rabea, A. S. Yassin, A. F. Mohammed, and ..., “Immunological characterization of the chemically prepared ghosts of Salmonella Typhimurium as a vaccine candidate,” BMC Veterinary …, 2022, doi: 10.1186/s12917-021-03112-4.
A. Muhammad, J. Champeimont, U. B. Mayr, and ..., “Bacterial ghosts as carriers of protein subunit and DNA-encoded antigens for vaccine applications,” Expert review of …, 2012, doi: 10.1586/erv.11.149.
N. Vinod et al., “A Salmonella typhimurium ghost vaccine induces cytokine expression in vitro and immune responses in vivo and protects rats against homologous and …,” PLoS …, 2017, [Online]. Available: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0185488
A. A. Amro, A. J. Neama, A. Hussein, E. A. Hashish, and ..., “Evaluation the Surface Antigen of the Salmonella typhimurium ATCC 14028 Ghosts Prepared by ‘SLRP,’” The Scientific World …, 2014, [Online]. Available: https://www.hindawi.com/journals/tswj/2014/840863/abs/
J. C. Garber, “Guide for the Care and Use of Laboratory Animals: National Research Council,” The National Academies Press, Washington, DC. https …, 2011.
A. A. Jabbar, I. A. A. Ibrahim, F. O. Abdullah, K. F. Aziz, and ..., “Chemopreventive Effects of Onosma mutabilis against Azoxymethane-Induced Colon Cancer in Rats via Amendment of Bax/Bcl-2 and NF-κB Signaling …,” Current Issues in …, 2023, [Online]. Available: https://www.mdpi.com/1467-3045/45/2/57
E. Nascimento-Gonçalves, B. A. L. Mendes, and ..., “Animal models of colorectal cancer: from spontaneous to genetically engineered models and their applications,” Veterinary …, 2021, [Online]. Available: https://www.mdpi.com/2306-7381/8/4/59
A. I. Thaker, A. Shaker, M. S. Rao, and M. A. Ciorba, “Modeling colitis-associated cancer with azoxymethane (AOM) and dextran sulfate sodium (DSS),” JoVE (Journal of Visualized …, 2012, [Online]. Available: https://www.jove.com/t/4100/modeling-colitis-associated-cancer-with-azoxymethane-aom-dextran
R. P. Bird, “Observation and quantification of aberrant crypts in the murine colon treated with a colon carcinogen: preliminary findings,” Cancer Lett, 1987, [Online]. Available: https://www.sciencedirect.com/science/article/pii/0304383587901571
J. D. Bancroft and M. Gamble, Theory and practice of histological techniques. books.google.com, 2008. [Online]. Available: https://books.google.com/books?hl=en&lr=&id=Dhn2KispfdQC&oi=fnd&pg=PR13&dq=theory+and+practice+of+histological+techniques+7+th&ots=JCjDcCUBD6&sig=-j7qWpcH8WtJauoPNbm4EGQaOl8
W. Yu, J. Zhang, Z. Chen, S. Wang, C. Ruan, and ..., “Inhibitory effect of a microecological preparation on azoxymethane/dextran sodium sulfate-induced inflammatory colorectal cancer in mice,” Frontiers in …, 2020, doi: 10.3389/fonc.2020.562189.
V. F. Machado, M. R. Feitosa, J. J. R. Rocha, and ..., “A review of experimental models in colorectal carcinogenesis,” Journal of Coloproctology …, 2016, [Online]. Available: https://www.scielo.br/j/jcol/a/hy9zQ7fVT5fV7PpmpHDzgHG/
K. Vdoviaková, E. Petrovová, M. Maloveská, and ..., “Surgical anatomy of the gastrointestinal tract and its vasculature in the laboratory rat,” … research and practice, 2016, [Online]. Available: https://www.hindawi.com/journals/grp/2016/2632368/abs/
S. S. Arango-Varela, I. Luzardo-Ocampo, and ..., “Andean berry (Vaccinium meridionale Swartz) juice in combination with Aspirin modulated anti-inflammatory markers on LPS-stimulated RAW 264.7 macrophages,” Food research …, 2020, [Online]. Available: https://www.sciencedirect.com/science/article/pii/S0963996920305664
M. M. Derry, K. Raina, R. Agarwal, and C. Agarwal, “Characterization of azoxymethane-induced colon tumor metastasis to lung in a mouse model relevant to human sporadic colorectal cancer and evaluation of grape …,” Experimental and Toxicologic …, 2014, [Online]. Available: https://www.sciencedirect.com/science/article/pii/S0940299314000293
F. Gossé, S. Guyot, S. Roussi, A. Lobstein, and ..., “Chemopreventive properties of apple procyanidins on human colon cancer-derived metastatic SW620 cells and in a rat model of colon carcinogenesis,” …, 2005, [Online]. Available: https://academic.oup.com/carcin/article-abstract/26/7/1291/2390898
W. Li and C. B. Li, “Lack of inhibitory effects of lactic acid bacteria on 1, 2-dimethylhydrazine-induced colon tumors in rats,” World J Gastroenterol, 2003, [Online]. Available: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4656522/
R. Suzuki, H. Kohno, S. Sugie, H. Nakagama, and ..., “Strain differences in the susceptibility to azoxymethane and dextran sodium sulfate-induced colon carcinogenesis in mice,” …, 2005, [Online]. Available: https://academic.oup.com/carcin/article-abstract/27/1/162/2390964
R. L. Johnson and J. C. Fleet, “Animal models of colorectal cancer,” Cancer and Metastasis Reviews, 2013, doi: 10.1007/s10555-012-9404-6.
M. Al-Obaybidi, Study the Effect of Lactobacillus acidophilus Local Isolate and its Components as Anticancer in vitro and in vivo. PhD thesis. Genetic Engineering …, 2013.
D. Kocijancic, S. Leschner, S. Felgner, R. M. Komoll, and ..., “Therapeutic benefit of Salmonella attributed to LPS and TNF-α is exhaustible and dictated by tumor susceptibility,” Oncotarget, 2017, [Online]. Available: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5482671/
C. H. Lee, C. L. Wu, and A. L. Shiau, “Toll-like Receptor 4 Mediates an Antitumor Host Response Induced by Salmonella choleraesuis,” Clinical Cancer Research, 2008, [Online]. Available: https://aacrjournals.org/clincancerres/article-abstract/14/6/1905/196343
S. Grille, M. Moreno, T. Bascuas, J. M. Marqués, and ..., “Salmonella enterica serovar Typhimurium immunotherapy for B‐cell lymphoma induces broad anti‐tumour immunity with therapeutic effect,” …, 2014, doi: 10.1111/imm.12320.
Y. Barak, F. Schreiber, S. H. Thorne, C. H. Contag, and ..., “Role of nitric oxide in Salmonella typhimurium-mediated cancer cell killing,” BMC Cancer, 2010, doi: 10.1186/1471-2407-10-146.
S. Kaimala, Y. A. Mohamed, N. Nader, J. Issac, and ..., “Salmonella-mediated tumor regression involves targeting of tumor myeloid suppressor cells causing a shift to M1-like phenotype and reduction in suppressive …,” Cancer Immunology …, 2014, doi: 10.1007/s00262-014-1543-x.
C. H. Lee, J. L. Hsieh, C. L. Wu, H. C. Hsu, and ..., “B cells are required for tumor-targeting Salmonella in host,” Applied microbiology and …, 2011, doi: 10.1007/s00253-011-3386-0.
C. H. Lee, J. L. Hsieh, C. L. Wu, P. Y. Hsu, and A. L. Shiau, “T cell augments the antitumor activity of tumor-targeting Salmonella,” Applied microbiology and …, 2011, doi: 10.1007/s00253-011-3180-z.
I. J. Lafta, “Crude Anthrax Protective Antigen Enhances Immunity For Salmonella Typhimurium in Mice,” J Fac Med Baghdad, 2017, [Online]. Available: https://www.iqjmc.uobaghdad.edu.iq/index.php/19JFacMedBaghdad36/article/view/133
Published
2024-04-26
How to Cite
Al-Qarraawi, R., & Al-Awade, H. A. R. (2024). Improved Histological Effect of Salmonella Typhimurium Ghost Vaccine Used in the Treatment of Colon Cancer Induced in Male Albino Rats. Central Asian Journal of Medical and Natural Science, 5(2), 172-185. https://doi.org/10.17605/cajmns.v5i2.2411
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