Immunological and Molecular Evaluation of miRNA-146a in Asthma Patients
Abstract
Asthma cause airway obstruction and are associated with chronic airway inflammation.
Abnormal immune response to environmental stimuli has caused many cells of the innate and
adaptive immune system to act. miRNAs play an important role in regulating key pathogenic
mechanisms in allergic inflammation, including polarization of adaptive immune responses and
activation of T cells. Material and Method: A case-control study and the implementation steps are
divided into 3 main parts, include: Patient selection and sampling, examining the intensity of gene
expression. Result: The present study show that the expression of miRNA-146a gene has been
decrease significantly in asthma. Regarding the intensity of miRNA-146a gene expression in the
patient and control groups, a significant difference can be observed between the two groups in terms
of gene expression intensity (P-Value=0.0001). Conclusion: The results of the present study show
that miR-146a gene expression has been significantly changed in asthma. In terms of the intensity of
miR-146a gene expression in the patient and control groups, a significant difference is observed
between the two groups (P-Value=0.0001), also the experiment and analysis of the obtained ROC
curve shows that a significant change mir-146a in Asthma.
References
Barnes PJ, Drazen JM. Pathophysiology of Asthma and COPD. 2002:343-59.
Lambrecht BN, Hammad H. The immunology of asthma. Nature Immunology. 2015;16(1):45-56.
Asher I, Haahtela T, Selroos O, Ellwood P, Ellwood E, Group GANS. Global Asthma Network survey suggests more national asthma strategies could reduce burden of asthma. Allergologia et immunopathologia. 2017;45(2):105-14.
Mims JW, editor Asthma: definitions and pathophysiology. International forum of allergy & rhinology; 2015: Wiley Online Library.
Maurya V, Gugnani HC, Sarma PU, Madan T, Shah A. Sensitization to Aspergillus Antigens and Occurrence of Allergic Bronchopulmonary Aspergillosis in Patients With Asthma. Chest. Greene CM, Gaughan KP. microRNAs in asthma: potential therapeutic targets. Current opinion in pulmonary medicine. 2013;19(1):66-72.
Boulet L-P, Reddel HK, Bateman E, Pedersen S, FitzGerald JM, O'Byrne PM. The global initiative for asthma (GINA): 25 years later. European Respiratory Journal. 2019;54(2):1900598.
Lin H, Zhang W, Zhou T, Li W, Chen Z, Ji C, et al. Mechanism of microRNA-21 regulating IL-6 inflammatory response and cell autophagy in intervertebral disc degeneration. Experimental and therapeutic medicine. 2017;14(2):1441-4.
Filipowicz W, Bhattacharyya SN, Sonenberg N. Mechanisms of post-transcriptional regulation by microRNAs: are the answers in sight? Nature reviews genetics. 2008;9(2):102-14.
Svitich O, Sobolev V, Gankovskaya L, Zhigalkina P, Zverev V. The role of regulatory RNAs (miRNAs) in asthma. Allergologia et Immunopathologia. 2018;46(2):201-5.
Zhang Y, Xue Y, Liu Y, Song G, Lv G, Wang Y, et al. MicroRNA-146a expression inhibits the proliferation and promotes the apoptosis of bronchial smooth muscle
cells in asthma by directly targeting the epidermal growth factor receptor. Experimental and Therapeutic Medicine. 2016;12(2):854-8.
Michaeloudes C, Abubakar-Waziri H, Lakhdar R, Raby K, Dixey P, Adcock IM, et al. Molecular mechanisms of oxidative stress in asthma. Molecular Aspects of Medicine. 2022;85:101026.
Aysola RS, Hoffman EA, Gierada D, Wenzel S, Cook-Granroth J, Tarsi J, et al. Airway remodeling measured by multidetector CT is increased in severe asthma and correlates with pathology. Chest. 2008;134(6):1183-91.
Laanesoo A, Urgard E, Periyasamy K, Laan M, Bochkov YA, Aab A, et al. Dual role of the miR‐ 146 family in rhinovirus‐induced airway inflammation and allergic asthma exacerbation. Clinical and translational medicine. 2021;11(6):e427.
Lu TX, Rothenberg ME. Diagnostic, functional, and therapeutic roles of microRNA in allergic diseases. Journal of Allergy and Clinical Immunology. 2013;132(1):3-13.
