The Usefulness and Discrimination Power of DYS391 Locus Using Power Plex ® Fusion and Power Plex®23Y Systems in Paternity Testing

  • Muna I. Hadi Pharmacist/M.Sc./Mass Graves Department /MLD/MOH
  • Hanan Kh. Mahmood Biology M.B.Ch.B./M.Sc., Head of Paternity and Kinship Sector/ Medical Legal Directorate (MLD)/ Ministry of Health (MOH)/Iraq
  • Khaleef M. Salih B.Sc./ Paternity and Kinship Sector/MLD/MOH
  • Dina M. Jameel B. Path. Analytics/ Paternity and Kinship Sector/MLD/MOH
Keywords: Paternity testing, male lineage, DYS391 locus, Power plex ®fusion system, Power plex ®Y23system, Power of Discrimination

Abstract

Thirty random cases of Iraqi families contain data of father/son DNA haplotypes have
been analyzed between 2017-2022 to find out the usefulness of DYS 391 Locus in paternity testing
using power-plex ®fusion and power-plex ®Y23systems. Scientifically, all cases were confirmed
and officially reported as an absolute cases of exclusion paternity. A combination of the two systems
should permit effective analysis with sufficient diversity and discrimination power of the locus. The
results show significant match (i.e., inclusion) at the DYS391 locus between alleged fathers and
corresponding sons for both kits in all the cases meanwhile there were no match (i.e., exclusion) in
many autosomal DNA loci and the rest Y-haplotypes other than DYS391. Furthermore, the results
revealed only three DYS391 allele polymorphisms; Allele number 10,11and 9 in a percentage of
69.23%, 27.692% and 3.076% contained in all 30 cases respectively. Allele frequencies, homozygosity,
haplotype diversity (HD), match probability and the power of discrimination of DYS391 locus (PD)
were estimated. The observed allele frequencies were (0.692, 0.276 and 0.030) for allele numbers 10,
11 and 9 respectively. Homozygosity was found to be (0.478, 0.0761 and 0.0009) respectively too.
Measurement of the upper bound confidence interval (CI) for each corresponding allele was (2.642,
2.234 and 1.989). Haplotype diversity was (0.451) and combined power of discrimination for DYS391
Locus was (0.4442). As a conclusion, there is a limitation in certifying DYS391 locus as an exclusive
locus useful Y-STR in testing paternity and male lineage in autosomal STR kits because of little allele
variants, poor power of discrimination and gene diversity.

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Published
2024-02-19
How to Cite
Hadi, M. I., Mahmood, H. K., Salih, K. M., & Jameel, D. M. (2024). The Usefulness and Discrimination Power of DYS391 Locus Using Power Plex ® Fusion and Power Plex®23Y Systems in Paternity Testing. Central Asian Journal of Medical and Natural Science, 5(1), 638-648. Retrieved from https://cajmns.centralasianstudies.org/index.php/CAJMNS/article/view/2388
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