PURIFICATION OF BIOACTIVE COMPOUNDS FROM STREPTOMYCES ISOLATES AND THEIR ANTIMICROBIAL ACTIVITY AGAINST SOME BACTERIA ISOLATED FROM FISH
Abstract
Antibiotic resistance (AR) is one of the major health problems that challenges successful treatment of various types and degrees of infection, both in humans and animals. According to that, it is critically important to discover natural new compounds to control AR. The presented herein-study was conducted to extract bioactive compounds from some isolates of Streptomyces and to evaluate their antibacterial activity on some bacterial microorganisms isolated from fish. The work started with the use of river sediments as a source for the isolation of Streptomyces bacteria, which were identified further with the recruitment of PCR that targeted the 16S rRNA gene. Then, samples of fish kidney, intestines, gills, and skin were utilized at 1 gm each to isolate some bacteria. Bioactive compounds were alcoholic-based-extracted from Streptomyces isolates and further investigated using GC-MS assay. These bioactive chemicals were evaluated as antibacterial agents against the fish bacterial isolates by employing Mueller Hinton agar (MHA). The results revealed two isolates of Streptomyces sp. mqw1 (SM1) and Streptomyces sp mqw2 (SM2). The fish isolates were Vibrio sp., Aeromonas sp., E. coli, Enterobacter sp., Pseudomonas sp., Staphylococcus sp., Klebsiella sp., Salmonella sp., and Serratia sp. The findings of MHA demonstrated higher antibacterial activities from SM2 isolates when compared with those from SM1. The findings of the current study indicate important antibacterial activities of the bioactive compounds, which were isolated from Streptomyces, especially mqw2, against fish bacterial isolates.
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