PHENOTYPIC DIAGNOSIS OF LABORATORY CULTURE OF FUNGI ISOLATED FROM THE EXTERNAL EAR CANAL EXTERNAL AUDITORY CANAL CAUSING OTITIS EXTERNA

Khalid Mohammed Abd; Ahmed Hamed Mahde Shugran

doi:10.17605/cajmns.v5i1.2304

Khalid Mohammed Abd Dept. of Biology - College of Education for Pure Sciences, University of Tikrit, Tikrit/Iraq
Ahmed Hamed Mahde Shugran Dept. of Biology - College of Education for Pure Sciences, University of Tikrit, Tikrit/Iraq

DOI: https://doi.org/10.17605/cajmns.v5i1.2304

Keywords: EAC Otomycosis, Candida spp, Aspergillus spp, Penicillium, Rhizopus

Abstract

69 isolates of fungi causing fungal external otitis EAC Otomycosis were obtained from patients with fungal external otitis 76.66% of the total number of 90 otological smears, 17 isolates of which belong to the genus Candida spp, 27 isolates belong to the genus Aspergillus spp, 4 co-infections of the said sexes, 5 isolates belong to the genus Alternaria, and 9 isolates belong to the genus penicillium, 6 isolates belonging to the genus Fusarium, and 5 isolates belonging to the genus Rhizopus all fungi were diagnosed by traditional methods through phenotypic examinations on a petri dish as well as the study of microscopic traits, so candida albicans and Aspergillus niger is the most visible fungus species in this study.

References

1. Prahan, B.; N. R, Tuladhar; and Amaty, R.M. (2003). preventation of otomycosis in outpatient department of Otolarengology in Tribuvan Univercity Teaching Hospital. Kathmanndu, Nepal. Ann. Otol Rhinol. Laryngol., 112: 384-387, cited by Mahmoudabdi (2006).
2. Paulose, K.O.; S., Al-Kalifa; p. Shenoy; J.Laryngol; and Sharma, R.k. (1989). Mycotic infection of the ear (Otomycosis): Aprospective study. Otol., 103 :30-35.
3. Kennedy, c.t.c. (1998). The external ear. (chap.68). in: Textbook of dermatology 6th (ed.) edited by Chamin, R.H. (ed.)pp.3031.
4. Mayer, G. and Heckel, A. (2006), Biologically Active Molecules with a “Light Switch”. Angewandte Chemie International Edition, 45: 4900 4921.
5. Leanor D. Haley, (1950). (August) III. Observations on Attempts to Induce Otomycosis in Rabbits. Author Affiliations Arch Otolaryngol. 1950; 52 (2): 214-219.
6. Shehab, C.M. (2005). Otomycosis clinco microbiological study in mosul Bord thesis. Submitted to Council of Otolarenology.
7. Ismail, L.D., El-Azizi, M.M., Khalifa, T.I. and Stermitz, F. R. (1995). derivatives and iridoid Glycosides from Penstemon crandallii Phyto-chemistry. 39(6):1391- 1393.
8. Jones, H. E.; Reinhardt, J. H. and Rinaldi, M. G. (1974). Immunological susceptibility to chronic dermatophytosis. Arch. Dermatol., 110: 213-220.
9. Kennedy, c.t.c. (1998). The external ear. (chap.68). in: Textbook of dermatology 6th (ed.) edited by Chamin, R.H. (ed.)pp.3031.
10. Linstrom, C. J ;Lucent, F.E.; Joseph, E.M.; And Baileys, B.J. (1988).Head and Neck surgery otolaryngology. 3 rd (ed). Lippincott William and William and Willikins, Philadelphia, pp.1711- 1724.
11. Harley, J.P., & Prescott, L.M. (1996). Laboratory exercises in microbiology.3rd ed. WCB /McGraw-Hill.
12. Emmons, C.W.; Binford, C.H. and Utz, J.P. (1974). Candidasis.In medical mycology. Lea and Fibiger ed.2nded. Philadelphia. Ch.,14 :167-182.
13. Baron, E. J.; Peterson, L. and Finegold, S. M. (1994). BaileyandScott’s ”Diagnostic Microbiology”, 9th ed. Hoffman Press, USA.
14. Larone, D. H. (1995). Medically important fungi: aguide and identification(3th Ed.) Asm. Press, washtngton, D.C. (Abst.).
15. Manns, J.M., Mosser, D.M., Buckley, H.R. (1994). Production of a haemolytic factor by Candida albicans Infection and Immunity, 62 (11) pp. 5154-5156.
16. Price, M. F., Wilkinson, I. D., & Gentry, L. O. (1982). Plate method for detection of phospholipase activity in Candida albicans. Sabouraudia: Journal of Medical and Veterinary Mycology, 20(1), 7-14.
17. Aubaid, Adnan & Muhsin, Tawfik & Al-Duboon, A.H. (1997). Extracellular enzyme activities of dermatophytes and yeast isolates on solid media. Mycoses. 40. 465-9. 10.1111/j.1439-0507.1997.tb00186.x.
18. Kannan, P., Janaki, C., & Selvi, G. S. (2006). Prevalence of dermatophytes and other fungal agents isolated from clinical samples. Indian journal of medical microbiology, 24(3), 212.
19. Champion, R. H., Burton, J. L., Burns, D. A., & Breathnach, S. M. (1998). Text book of Dermatology. 6th ed. Black well science Ltd. pp 1277- 1376.
20. Pontes, Z. B. V. D. S., Silva, A. D. F., Lima, E. D. O., Guerra, M. D. H., Oliveira, N. M. C., Carvalho, M. D. F. F. P., & Guerra, F. S. Q. (2009). Otomycosis: a retrospective study. Brazilian Journal of otorhinolaryngology, 75, 367-370.‏
21. Rancic, N. K., Miljkovic, P. M., Deljanin, Z. M., Marinkov-Zivkovic, E. M., Stamenkovic, B. N., Bojanovic, M. R.,.. & Otasevic, S. A. (2022). Knowledge about HPV Infection and the HPV Vaccine among Parents in Southeastern Serbia. Medicina, 58(12), 1697.‏
22. Ahmad, Y., Ahmad, M. N., Zia, A., Alam, S. S., Khan, R. A. A., & Riaz, M. (2020). Biocontrol of economically important weed species through endophytic fungi isolated from Parthenium hysterophorus (Family: Asteraceae). Egyptian Journal of Biological Pest Control, 30(1), 1-8.‏
23. Gharaghani, M., Jafarian, H., Hatami, M., Shabanzadeh, M., & Mahmoudabadi, A. Z. (2022). Evaluation of catalase activity of clinical and environmental isolates of Aspergillus species. Iranian Journal of Microbiology, 14(1), 133.‏
24. Gupta, K. L., Bagai, S., Ramachandran, R., Kumar, V., Rathi, M., Kohli, H. S.,.. & Chakrabarti, A. (2020). Fungal infection in post-renal transplant patient: Single-center experience. Indian Journal of Pathology and Microbiology, 63(4), 587.‏
25. AL-TAEE, M. M., AL-TARBOOLEE, A. Y., & HADI, W. H. (2020). A Study of Anti-Fungal Activity for Some Antibiotics Against Aspergillus spp. and Candida spp. in Iraqi patients with Diabetes Mellitus. International Journal of Pharmaceutical Research, 12(4).‏
26. Aboutalebian, S., Mahmoudi, S., Mirhendi, H., Okhovat, A., Abtahi, H., & Chabavizadeh, J. (2019). Molecular epidemiology of otomycosis in Isfahan revealed a large diversity in causative agents. Journal of medical microbiology, 68(6), 918-923.