INVESTIGATION OF THE EFFECT OF CHITOSAN NANOPARTICALS ON MDR BACILLUS CEREUS ISOLATED FROM PASTEURIZED MILK

Aenas laith  ali; Aenas laith  ali; Marwa Fadhil  Alsaffar; Hieam adnan  ali; Solav. A.  albakery; Mohammed Sabah  Otaiwi

doi:10.17605/cajmns.v5i1.2302

Aenas laith ali Department of Physiology, Hammurabi College of Medicine, University of Babylon, Iraq
Aenas laith ali Department of Physiology, Hammurabi College of Medicine, University of Babylon, Iraq
Marwa Fadhil Alsaffar Assist Prof. AL-Qasim Green University \Iraq, Hillah, Babil, Iraq
Hieam adnan ali Department of Physiology, Hammurabi College of Medicine, University of Babylon, Iraq
Solav. A. albakery Department of Physiology, Hammurabi College of Medicine, University of Babylon, Iraq
Mohammed Sabah Otaiwi Department of Science, College of Basic Education, University of Babylon, Babylon, Iraq

DOI: https://doi.org/10.17605/cajmns.v5i1.2302

Keywords: Nanoparticles, caritosan, pasteurized milk

Abstract

The current study focused on extending the storage life of pasteurized milk by taking advantage of nanoparticles of the biopolymer known as chitosan, where the nanoparticles were synthesized based on a green technology, meaning that it does not have any negative harm to the synthetic material. The laboratory-synthesized nanoparticles were characterized by a size ranging from 100 to 140 nanometers when examined immediately after the anthesis and the size when examined two weeks after the anesthesia, the size was 115-220 nm, using a transmission electron microscope (TEM) of the type Philips CM10. By studying the inhibitory action of the nanoparticles in the Muller-Hinton agar culture medium, it was found that the synthesized particles have the ability to combat the pathogenic Bacillus cereus bacteria that form blackboards when using different concentrations (0. 25 - 0. 5 - 1%), where it was found that the best diameter of the contrast corona was (14-15 mm at the two concentrations (0. 5) (1) sequentially When testing the biological validity of pasteurized milk at a temperature of 5 semen for a period of -1-15-30 days of storage and after adding nano-chitosan using the three aforementioned concentrations, it was found that the number of B. cereus It was varied as it reached on the first day when using the three concentrations 10 FU/ml 1 x, but on the fifteenth day the numbers were as follows 10 CFU/ml 2 x 10 -3 x 10 - 5 x and according to the concentrations (0. 25) - 0. 5 - 1% On the thirtieth day, the bacterial numbers decreased in pasteurized milk fortified with nanoparticles of chitosan, and the result was as follows (10 CFU/mL (10) - 10 - 2 x relative to the three concentrations, respectively). This result was an indication of the effectiveness of nanoparticles of chitosan in preventing cell growth. vegetation and the formation of new spores compared with the number of Bacillus cereus, which is approximately 107 CFU/ml x when pasteurized milk is stored for 15 days at a temperature of 5°C. It indicates contamination of milk and its products. It has been observed that increasing the concentration of added nano-chitosan increases the antimicrobial activity.

References

1. Aranaz, I. ; Mengibar, M. ; Harris, R. ; Panos, I. ; Miralles, B. ; Acosta, N. ; Galed, G. and Heras, A. (2009). Functional characterization of chitin and chitosan. Current Chemical Biology, 3: 203-230
2. Dutta, P. K. ; Tripathi, S. ; Mehrota, G. K. ; Du a, J. (2009). Prespec ves for chitosan based antimicrobial films in food applications, Chemistry. , 114: 1173-1182
3. Barry, A. O. and Marcel, I. (2013). Solid state Fermentative Production and Bioactivity OF Fungal Chitosan, International peer-reviewed scientific online journal 3(2):172-175
4. Dina, Raafat Gouda Fouad. (2008). Chitosan an as compound:Modes of action and resistance mechanisms. Dissertation submitted to Rheinischen Friedrich-wilhlms-universität Bonn
5. Andres, Y. ; Giraud, L. ; Gerente, C. and Le Cloirec P. (2007). Antibacterial effects of chitosan powder Mechanisims of action. Environmental Technology (28)
6. Akila, R. M. (2014). Fermentative production of fungal Chitosan, a versatile biopolymer (perspectives and its applications), Advances in Applied Science Research, 5(4): 157-170.
7. Franco, L. O. ; Stamford, T. C. M. ; Stamford, N. P. ; Campos-Takaki, G. M. (2005). Cunningamella elegans (IFM 46109) como fonte de quitina e quitosana. Rev. Anal. , 14
8. Crestini, C. ; Kovac, B. and Giovannozzi-Sermanni, G. (1996). Production and Isolation of chitosan by submerged and solid-state fermentation from Lentinus edodes. Bioengineering 50: 207-210
9. Akhilesh, V. Singh. (2011). Biopolymers in Drug Review. Pharmacologyonline Newsletter, 1: 666-674
10. Devlieghere, F. , A. Vermeulen, and J. Debevere. (2004). Chitosan: antimicrobial activity, interactions with food components and applicability as a coating on fruit and vegetables. Food Microbiol. 21:703-714
11. Horst, M. N. ; Walker, A. N. and Klar, E. (1993). The pathway of crustacean chitin synthesis. In The crustacean integument: Morphology and biochemistry. Horst, M. N. J., Freeman, A. Eds. ; CRC: Boca Raton, FL, 113-149
12. Guibal, E. (2004). Interactions of metal ions with chitosan-based sorbents. Separation and Purification Technology, 38(1): 43-74
13. Bhargava, R. , Wang, S. , & Koening, J. L. (2003). FTIR Microspectroscopy of polymeric systems, Advances in Polymer Science, 163: 137-191
14. Cho, Y. I. No, H. K. ; Meyers, S. P. (1998). Physicochemical characteristics and functional properties of various commercial chitin and chitosan products, Journal of Agricultural and food Chemistry, 46: 3839-3843
15. Je, J. Y. & Kim, S. K. (2006). Chitosan derivatives killed bacteria by disrupting the outer and inner membrane Journal of agricultural and food Chemistry 54
16. Begin, A. and Calsteren, M. R. (1999). Antimicrobial films produced from chitosan. International Journal of Biological Macromolecules. 26: 63-67
17. Jolanta, K. ; Malgorzata, C. ; Zbigniew, K. ; Anna, B. ; Krzystof, B. ; Jorg, 6629-6633. T. and Piotr, S. (2010). Application of Spectroscopic Methods for Structural Analysis of Chitin and Chitosan. Marine Drugs. 8:1567-1636.
18. Dutta, P. K. ; Ravikumar, M. N. V. and Dutta, J. (2002). Chitin and Chitosan for versatile applications. Journal of macromolecular science. 42: 307
19. Hirano, S. ; Sato, N. ; Yoshida, S. and Kitagawa, S. (1987). Chemical modification of chitin and chitosan, and their novel applications. In "Industrial Polysaccharides: Genetic Engineering, Structure/Property Relations and Applications, " M. Yalpani (Ed. ), . 163-176
20. Dawn, C. P. ; Kalaivani, N. ; Abdul Jalil, A. (2004). Production and quality of chitosan extracted from local fungal isolates, The 4th Annual Seminar Of National Science Fellowship.
21. Isa, M. T. ; Ameh, A. O. ; Tijani, M. and Adama, K. K. . (2012). Extrac on and characterization of chitin and chitosan from Nigerian shrimps, International Journal of Biological and Chemical Sciences. 6(1): 446-453
22. Li, Q. , Dunn, E. T. ; Grandmaison, E. W. and Goosen, M. F. A. (1992). Applications and properties of chitosan. journal of Bioactive and Compatible Polymers. 7: 370-397.
23. Kannan M. ; Nesakumari, M. ; Rajarathinam, K. and Singh, A. (2010). Production and Characterization of Mushroom chitosan under solid state fermentation conditions Advances in Biolgical Research, 4(1):10-13
24. Logesh AR ;Thillaimaharani, K. A; Sharmila, K. ; Kalaiselvam, M. and Raffi, S. M. (2012). production of chitosan from endolichenic fungi isolated from mangrove environment and its antagonistic activity, Asian Pacific Journal of Tropical Biomedicine. 140-143
25. Madigan, M. T. ; Martinko, J. M. and Parker J. (2000). (Eds), (Upper Saddle River, N. J. Prentice Hall), 101-110
26. Li, Q. ; Dunn, E. T. ; Grandmaison, E. W. and Goosen, M. F. A. (1992). Applications and properties of chitosan. journal of Bioactive and Compatible Polymers. 7: 370-397
27. Mohammad, A. E. ; Aroona, C. ; Eshrat, G. - Fathabad and Fereshteh, P. (2013). Preparation of Chitossan from Pencillium SPP. And Determination of their degree of deacetylation. Indian Journal of Biotechnology. 12:231-235
28. Muzzarelli, R. A. A. (1977). Enzymatic synthesis of chitin and chitosan. Occurrence of chitin. In: Chitin (Muzzarelli, R. A. A. , ed. ) 5-44. Pregamon Press, New York, NY.
29. Nessa, F. ; Shah, M. M. ; Asaduzzaman, M. ; Roy, S. K. ; Hossain, M. M. & Jahan, M. S. (2010). Aprocess for the preparation of Chitin and Chitosan from and Prawn shell waste. Bangladesh Journal of Scientific and Industrial Research, 45(4): 323-330
30. No, H. K. and Meyers, S. P. (1995). Preparation and characterization of chitin and chitosan-A Review. Journal of Aquatic Food Product Technology. 4(2) 27-52. Archana R. Juvekar. (2014). Fermentative
31. Pradnya N. Vaingankar and Production of Mycelial Chitosan from Zycomycetes:Media Optimization and Physico-chemical Characterization, Advance in bioscience and biotechnology (5): 940-956.
32. Shigemasa, Y. ; Minami, S. (1996). Applications of chitin and chitosan for biomaterials. Biotechnology & genetic engineering reviews, 17: 383-420.
33. Yen, M. T;yang, j. h. and Mau, J. L. (2008). Antioxidant properties of chitosan from carb shells. carbohydrate. polymers. 74: 840-844.
34. Zheng, L. Y. and Zhu, J. F. (2003). Study on antimicrobial activity of chitosan with different molecular weights. Carbohydrate. Polymers. 54: 527-530.