STUDY SOME VIRULENCE FACTORS AND ANTIBIOTIC SUSCEPTIBILITY OF SOME CANDIDA SPP. ISOLATED FROM DIFFERENT CLINICAL SITES
Abstract
The current study aimed to study some virulence factors and test the antibiotic susceptibility of some fungi isolated from different clinical sites. This study was conducted in the Postgraduate Studies Laboratory / College of Science - University of Kirkuk. (220) samples were collected from chronic wound infections for patients hospitalized in Kirkuk General Hospital and Azadi Teaching Hospital in the city of Kirkuk. Each group included (69) samples from bed sore patients and (62) Samples from surgical infections (58) samples from diabetic foot patients and (31) samples from burn patients in the period between September to November 2023. Three types of yeast were isolated and diagnosed: Candida albicans, Candida tropical, and Candida krusei, and the most frequent ones were It is the yeast C. albicans, with 28(50%), Candida tropical, 18(32%), and C. krusei, 10(18%). Regarding virulence factors, C. albicans yeast showed the ability to form germ tubes and chlamydial spores. Chlamydospore formation While C. krusei and C. tropicalis were able to form surface growth on SDB liquid medium, the types of yeast showed different colors by growing them on chromo agar, as C. albicans was light green and C. krusei was pink. Light while C. tropicalis yeast has a metallic blue color. Regarding the drug sensitivity test, the results showed that all types of yeast were sensitive to the antibiotic Nystatin, reaching the diameters of inhibition. As for Amphotericin, all types of yeast were sensitive to it, and it was the most effective antibiotic among all types, with a rate of 100%. On the other hand, it was observed that the types of yeast were 100% sensitive, with the exception of two isolates of the type C. krusei, which were resistant to the antibiotic Clotrimazole, and to the antibiotic Ketoconazole. All types showed sensitivity to this antibiotic, except for one isolate, the yeast C. krusei, which was resistant to this antibiotic.
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