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Introduction: Urinary tract infections (UTIs) are among the most common infections worldwide. Uropathogenic Escherichia coli (UPECs) are the main causative agent of UTIs. UPECs initially colonize the human host adhering to the bladder epithelium. Adhesion is followed by the bacterial invasion of urothelial epithelial cells where they can replicate to form compact aggregates of intracellular bacteria with biofilm-like properties.

Aim: Isolation and identification of Escherichia coli from urinary tract infection of children and antibiotic susceptibility test by disc diffusion method for Escherichia coli.

Materials & methods: 120 samples will collect from urine of urinary tract infection. These samples will identify on different culture media such as MacConkey agar their biochemical tests will examine. After identification, E. coli isolates will keep in LB broth supplemented with 20% glycerol at -20°C for further use.

Results: A total of 120 samples were taken from patients, with an average of 40(33.3%) samples for males and 80 (66.7%) for females. The results showed that 37(30.8%) of the samples showed positive E. coli growth. About antibiotic susceptibility test E. coli showed total resistance (100%) to Amoxicillin/clavulanic acid and Ampicillin. 50% resistance to Nalidixic. Otherwise, E. coli showed 40%, 20%, 60%, 20% and 50% resistance to Imipenem, Amikacin, Gentamicin, Azithromycin and Cefepime respectively.

Conclusions: The current study showed that Escherichia coli are one of the causative agents of urinary tract infection


UTIs E. coli antibiotic susceptibility Azithromycin Amikacin

Article Details

How to Cite
Balqees Ali Ridah, Dariush Minai-Tehrani, & Abdulmueen Shabeeb Hamad. (2024). ISOLATION AND IDENTIFICATION OF ESCHERICHIA COLI FROM CHILDREN WITH URINARY TRACT INFECTION IN KIRKUK CITY. Central Asian Journal of Medical and Natural Science, 5(1), 1-7.


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