ISOLATION AND IDENTIFICATION OF ESCHERICHIA COLI FROM CHILDREN WITH URINARY TRACT INFECTION IN KIRKUK CITY

Balqees Ali Ridah; Dariush Minai-Tehrani; Abdulmueen Shabeeb Hamad

doi:10.17605/cajmns.v5i1.2264

Balqees Ali Ridah Microbiology and Microbial Biotechnology Dept., Faculty of Biology Science & Biotechnology, Shahid, Iran
Dariush Minai-Tehrani Microbiology and Microbial Biotechnology Dept., Faculty of Biology Science & Biotechnology, Shahid, Iran
Abdulmueen Shabeeb Hamad Medicinal and Industrial Plant Department, College of Agriculture /Al-Hawija, University of Kirkuk, Iraq

DOI: https://doi.org/10.17605/cajmns.v5i1.2264

Keywords: UTIs, E. coli, antibiotic susceptibility, Azithromycin, Amikacin

Abstract

Introduction: Urinary tract infections (UTIs) are among the most common infections worldwide. Uropathogenic Escherichia coli (UPECs) are the main causative agent of UTIs. UPECs initially colonize the human host adhering to the bladder epithelium. Adhesion is followed by the bacterial invasion of urothelial epithelial cells where they can replicate to form compact aggregates of intracellular bacteria with biofilm-like properties.

Aim: Isolation and identification of Escherichia coli from urinary tract infection of children and antibiotic susceptibility test by disc diffusion method for Escherichia coli.

Materials & methods: 120 samples will collect from urine of urinary tract infection. These samples will identify on different culture media such as MacConkey agar their biochemical tests will examine. After identification, E. coli isolates will keep in LB broth supplemented with 20% glycerol at -20°C for further use.

Results: A total of 120 samples were taken from patients, with an average of 40(33.3%) samples for males and 80 (66.7%) for females. The results showed that 37(30.8%) of the samples showed positive E. coli growth. About antibiotic susceptibility test E. coli showed total resistance (100%) to Amoxicillin/clavulanic acid and Ampicillin. 50% resistance to Nalidixic. Otherwise, E. coli showed 40%, 20%, 60%, 20% and 50% resistance to Imipenem, Amikacin, Gentamicin, Azithromycin and Cefepime respectively.

Conclusions: The current study showed that Escherichia coli are one of the causative agents of urinary tract infection

References

1. Foxman, Betsy. “The epidemiology of urinary tract infection.” Natures review, 2010: 7,653–660.
2. Ana,F.,Walker,J.,C.,Hultgren,S. Urinary Tract infections; epidemiology, mechanisms of infection and treatment options.” Nature review microbiology, 2015: 57-61.
3. Najar, S., Saldahna, L.,Banday k.“Approach to urinary tract infections.” Indian journal of nephrology, 2009: 19(4): 129–139.
4. Brusch, John L. “Pathophysiology of Complicated Urinary Tract Infections.” medscape, 2017: 2039975.
5. Flores-Mireles, A., Pinkner, S., Caparon, G., & Hultgren J. “Avaccine antibodies block binding of Enterococcus faecalis to fibrinogen to prevent catheter-associated bladder infection in mice.” Science translational medicine, 2014: 6;254.
6. Pabich, W., Fihn, S., Stamm w., Scholes,D. ,Boyko,E. “Prevalance and determinants of vaginal flora alterations on post menosomal women.” journal of infectious diseases, 2003: 37(1),57.
7. Zhang S, Abbas M, Rehman MU, et al. Dissemination of antibiotic resistance genes (ARGs) via integrons in Escherichia coli: a risk to human health. Environ Pollut. 2020;266:1152–1160.
8. Al-Geobory, H. A. 2011. Comparative study between Methicillin resistant Staphylococcus aureus (MRSA) and Methicillin sensitive Staphylococcus aureus (MSSA), and detect the antimicrobial effects of some plant extracts on them. Msc. Thesis. College of Science/ Baghdad University. Iraq
9. Parvez, S. A., and Rahman, D. (2018). Virulence Factors of Uropathogenic E. coli. Microbiology of Urinary Tract Infections.
10. Sanocka-maciejewska D, Clupinska M, Kurpisz M. Bacterial infection and semen quality. J Reprod Immuno 2005; 67: 51-56.
11. Ibadin OK, Ibeh IN. Bacteriospermia and sperm quality in infertile male patient at University of Benin Teaching Hospital, Benin City, Nigeria. Mal J Microbiol 2008;4:65 7.
12. Golshani M, Taheri S, Eslami G, et al. Genital tract infection in asymptomatic infertile men and its effect on semen quality. Iranian J Publ Health 2006; 35(3): 81-84.
13. Fraczek, M. and Kurpisz, M.Mechanisms of the harmful effects of bacterial semen infection on ejaculated human spermatozoa: potential inflammatory markers in semen. Folia Histochem Cytobiol, 2015; 53:201–217.
14. Tremellen, K. Oxidative stress and male infertility--a clinical perspective. Hum Reprod Update, 2008; 14:243–258.
15. Moretti E., S. Capitani, N. Figura et al., “The presence of bacteria species in semen and sperm quality,” Journal of Assisted Reproduction and Genetics, vol. 26, no. 1, pp. 47–56, 2009.
16. Isaiah, I. N., Nche, B. T., Nwagu, I. G. and Nnanna, I. I. Current studies on bacteriospermia the leading cause of male infertility: a protege and potential threat towards man’s extinction. N. Am. J. Med. Sci., 2011; 3(12)562-564.
17. Al-Saadi, H.Q.B. and Abd, S.A. The Effect of Bacterial Infection on Male Infertility. Iraqi Journal of Biotechnology, 2019; Vol. 18, No. 3, 72-84.
18. Merino, G., Carranza-lira, S., Murrieta, S., Rodriguez, L., Cuevas, E.& Moran, C. Bacterial Infection and Semen Characteristics in Infertile Men, Archives of Andrology, 1995; 35:1,43-47,
19. Aminzadeh Z, Sadat M, Shabani M. Bacteriuria by ESBL producing E. coli and Klebsiella pneumoiae. Iranian Journal of Kidney Diseases 2008;2(4):197-200.
20. Sahm DF, Thorsberry C, Mayfeild DC, et al. MDR urinary tract isolates of E.coli, Prevention and patients demographics in the United States in 2000. Antimicrob Agents Chemoth. 2001; 35:236-239
21. Iroha I.R, Adikwu M.U, Esimone C.O, et al. ESBL in E. coli isolated from a tertiary hospital in Enugu state, Nigeria. Pak J Med Sci 2009; 25 (2): 279-282
22. Sasirekha B, Manasa R, Ramya P, et al. Frequency and antimicrobial sensitivity pattern of ESBL producing E. coli and Klebsiella pneumoniae isolated in a tertiary care hospital. Al Ameen J Med Sci 2010; 3(4):265- 271.
23. Jirachai W, Kamonwon J, Wacharee J. The prevalence and Susceptibility patterns of ESBL-producing Klebsiella pneumoniae and E. coli in Chonburi Hospital. J Infect Dis Antimicrob agents. 2006;23(2):57-63.
24. Daoud Z, Hakime N. Prevalence and susceptibility patterns of ESBL-producing E. coli and Klebsiella pneumoniae in a general hospital in Beiurut, Lebanon. Rev Esp Quimioterap, 2003; 16(2):233-238.
25. Chlebicki M. P, HML Oh. ESBL in clinical isolates of E. coli and Klebsiella spp. In a Singapore hospital. Annals Academy of Medicine 2004; 33(3): 302-306.
26. BabypadmIni S, Appalaraju B. ESBL in urinary isolates of E. coli and Klebsiella pneumoniae prevalence and susceptibility pattern in a tertiary hospital. IJMM 2004; 22(3):172-174.
27. Husam S.K, Khalid M. B, Abiola C. S, et al. ESBL in E. coli and Klebsiella pneumoniae. J Infect Dev Ctries 2009; 3(4):295-299.
28. Abdulla A K and Kumar A. Prevalence and antimicrobial susceptibility of ESBLproducing E. coli and Klebsiella pneumoniae in a general hospital. Ann Saudi Med 2005; 25(3):239-242.