FEATURES OF CHANGES IN IMMUNE HOMEOSTASIS IN PATIENTS WITH BRONCHIAL ASTHMA

Turdibekov Husan; Nizomov Bakhtiyor; Toshnazarov Shuxratillo; Ibragimov Sanjarbek; Jurabaeva Gulira'no

Turdibekov Husan Assistantof the department of Ftiziopulmonologii, Samarkand State Medical Institute Samarkand, Republic of Uzbekistan
Nizomov Bakhtiyor Assistant, department of internal medicine pediatric faculty, Samarkand state medical institute, g. Samarkand, Republic of Uzbekistan
Toshnazarov Shuxratillo Assistant, department of internal medicine pediatric faculty, Samarkand state medical institute, g. Samarkand, Republic of Uzbekistan
Ibragimov Sanjarbek Students, medical faculty, Djalalabad State University, Republic of Kyrgyzstan
Jurabaeva Gulira'no Students, pediatric faculty, Tashkent medical institute of Pediatrics

Keywords: bronchial asthma, Gln27Glu polymorphism of the β2-BArenoreceptor gene, cytokines IL-4, IFN-γ, IgE

Abstract

92 patients with bronchial asthma (BA) were examined. Among them, 48 patients with allergic BA (52.2%), 12 patients with non-allergic BA (NBA) (13%) and 28 patients with mixed BA (SBA) (26.8%). In 71 (77.2%) patients, the hereditary history was delayed. Determination of total IgE in blood serum was carried out by the ELISA method using a set of the company "Hema-Media", the level of IFN-γ, IL-4 in blood serum using test systems for quantitative determination by the solid-phase ELISA method (CJSC "Vector-Best", Russia). Genotyping of DNA samples was performed by PCR amplification.

The results obtained show a certain dependence of the levels of total IgE, IL-4, and IFN-g in patients with BA with a pathogenetic variant of the disease, the distinctness of which is more pronounced in patients with ABA. In patients with ABA and SBA, there is a pronounced inhibition of IFN-γ production, while in patients with BA with a predominantly allergic mechanism of the disease, a significant increase in the level of IL-4 is recorded. According to the results of our studies, there was no significant relationship between the Gln27Glu polymorphism of the β2-BArenoreceptor gene and the activity of inductive cytokines. The Gln27Gln genotype of the β2-BArenoreceptor gene in patients with BA and, especially, in the allergic form of the disease, is associated with IgE hyperproduction.

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