Polymorphism of acyl-CoA synthetase 1 ACSL1 Gene rs2292899 in Iraqi Patients with type2 Diabetes Mellitus

Manal Hassan  Foad; Dr. Intisar Hussein  Ahmed

Manal Hassan Foad Departmen of Biology, College of Education for pure Sciences, University of Wasit, Iraq
Dr. Intisar Hussein Ahmed Departmen of Biology, College of Education for pure Sciences, University of Wasit, Iraq

Keywords: ACSL1

Abstract

The aim of this study was to investigate the association of polymorphism of acyl-CoA synthetase 1 (ACSL1) rs2292899 to the susceptibility of type2 diabetes mellitus in diabetic patients from Wasit province using TaqMan SNP genotyping assay. A total of 80 participants (45 confirmed patients with type2DM and 35 healthy individuals as controls) were selected by using a convenient sampling method. The results of the current study displayed that in both in patients and control groups, the distribution frequencies of the genotypes and alleles of acyl-CoA synthetase 1 rs2292899 were according to Hardy Weinberg equilibrium P>0.05, indicating that the frequency of the gene has reached the genetic equilibrium and the selected samples were representative of the population. G allele is a major one in the studied groups. This allele is more frequent in T2DM patients (92.22%) compared to controls (84.29%). Two genotypes were detected in acyl-CoA synthetase 1 rs2292899.The homozygous GG genotype was the main genotype in studied group with higher percentage in T2DM patients 84.44% compared with 68.57% in controls, p=0.09166.The heterozygous AG genotype was lower in patients (15.56%) vs. (31.43%) in healthy controls. These results suggest that the G allele might be a risk factor in T2DM while the A allele may be considered as a protective allele against the disease. The association analysis showed that the GG genotype increases the association with T2DM than that in controls OR=2.4881 (CI95% [0.8478 to 7.3022]), p = 0.0970. The heterozygous genotype of AG decreases the association with T2DM OR=0.4019(CI95% [0.1369 to 1.1796]), p = 0.097.The subgroup analysis among females demonstrated that the genotypes GG or AG was not associated with T2DM with OR=1.00 for each genotype. OR of 1.00 indicates that the risk is comparable in the two groups. The association analysis showed that the GG genotype increases significantly T2DM risk among the males patients OR= 6.7083 (CI95% [1.2018 to 37.4450]), p = 0.0300. The heterozygous AG genotype decreases significantly the association with the disease OR= 0.1491 (CI95% [0.0267 to 0.8321]), p = 0.0300.In conclusion, the acyl-CoA synthetase 1 rs2292899 have possible roles in type2 diabetes mellitus susceptibility.

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