Antibacterial Activities of Cranberry Extract Against Isolated Bacteria from UTI Patients

Hiba abdullrazaq Naji Faisal Al-shamary; Haifaa Mahdi Hussain Al-janabi; Zaman Sameer Jassam Al-daamy

doi:10.17605/cajmns.v4i3.1571

Hiba abdullrazaq Naji Faisal Al-shamary Department of Satisfactory analyzes, College of applied medical science, University of karbala
Haifaa Mahdi Hussain Al-janabi Department of Satisfactory analyzes, College of applied medical science, University of karbala
Zaman Sameer Jassam Al-daamy Department of Satisfactory analyzes, College of applied medical science, University of karbala

DOI: https://doi.org/10.17605/cajmns.v4i3.1571

Keywords: Urinary Tract Infection, Cranberry, Antibacterial activity

Abstract

Methods: Cranberry was extracted by means of aqueous and alcohol extraction, in addition to the collection of bacteria samples from patients with urinary tract infection in Imam Hussein Medical city and Alhindyia Hospitals and re-diagnosis by traditional methods (biochemical tests). then testing the sensitivity of antibiotics and measuring the inhibitory effect of the cranberry extract of the bacterial species obtained during this study.

Results: The results of the current study showed that the re-culturing and identifying of bacterial samples obtained from patients with urinary tract infection have shown five bacterial strains (Echerichea Coli, Pseudomonas, Enterobacter, Protus, Staphylococcus aureus ). The study showed that the aqueous extract has the highest inhibitory effect against the bacteria and the diameter of the inhibition of 29 mm followed by the extract of methanol and the diameter of inhibition of 28 mm and then the plant Cranberry crude and diameter of inhibition was 19 mm. The sizes of inhibition zones were different according to concentration, We observe an increase in the diameter of the inhibition zone with increased concentration.

Conclusion: In conclusion the, extract of cranberry has antibacterial activity against uropathogens including E. coli, S aureus, Enterobacter, Pseudomonas and Proteus. Therefore.the potential of cranberry product to act as a non-antibiotic alternative for preventing UTI, thereby reducing the total amount of antibiotics prescribed for treatment of UTIs and preventing drug resistance.

References

1. Kaviya Srinidhi.A /J. Pharm. Sci. & Res. Vol. 6(1), 2014, 41 – 44.
2. Stamm WE (2001). Catheter-associated urinary tract infections: Epidemiology, pathogenesis, and prevention. Am. J. Med. 91: 65-71.
3. Bethesda MD (2005). National kidney and urologic diseases information clearing house. NIH Publication, No. 08– 3925. Biochemistry. 19th Ed.California: Lange Medical Publications. Pp.115-117.
4. David SH, Joseph AS, Francisco T, Richard S, John DH, Pamela LD (2008). Streotozocin-Induced diabetic mouse model of urinary tract infection.Infect. Immun. 76(9): 4290-4298.
5. Iroha IR, Adikwu MU, Esimone CO, Aibinu I, Amadi ES (2009). Extended spectrum Beta-Lactamase (ESBL) in E. coli isolated from a tertiary hospital in Enugu State, Nigeria. Pak. J. Med. Sci., 25(2): 279-282.
6. Ronald AR, Harding GK.Urinary infection prophylaxis in women, Ann Intern Med, 1981, vol.94 (pg.268-70).
7. GuptaK, ScholesD, StammWE.Increasing prevalence of antimicrobial resistance among uropathogens causing acute uncomplicated cystitis in women, JAMA, 1999, vol.281 (pg.736-8).
8. ReidG.Probiotic therapy and functional foods for prevention of urinary tract infections: state of the art and science, Curr Infect Dis Rep, 2000, vol.2 (pg.518-22).
9. Jepson RG, Craig JC (2008). Cranberries for preventing urinary tract infections. Cochrane Database Syst. Rev. 23: 2-8.
10. Mahon C, Manuselis G (2006). Textbook of diagnostic microbiology 3nd ed. Philadelphia, WB Saunders USA.
11. Quinn PJ, Markey BK, Carter ME, Donnelly WJ, Leonard FC (2004). “Veterinary Microbiology and Microbial Diseases Textbook”. Printed and bound in Great Britain by International Ltd. Mosby, London, Padstow-Cornwall. Pp. 118-126.
12. Mbata TI, Debiao LU, Saikia A (2008). Antibacterial activity of the crude extract of Chinese green tea (Camellia sinensis) on Listeria monocytogenes. Afr.J. Biotechnol. 7: 1571-1573.
13. Ajayi AO (2013). Impedimetric evaluation of the synergistic effects of oregano and cranberry aqueous extract mixtures on the growth of Salmonella typhimurium. MAPRJ. 1(2): 12-1.
14. Morello, J.A.; H.E. Mizer; &Granato. (2006). Laboratory Manual and Workbook in Microbiology Applications to Patient Care. 18th.ed. The McGraw-Hill Companies, Inc., New York: 95-99.
15. Egorove, N.S.(1985).Antibiotics ascientific approach. Mir Publishers, Moscow.
16. Magarinos HL, Sahr C, Selaive SD, Costa ME, Figuerola FE, Pizarro OA (2008). In vitro inhibitory effect of cranberry (Vaccinium macrocarpon Ait.) juice on pathogenic microorganisms. Prikl. Biokhim.Mikrobiol. 44: 333-336.
17. Cimolai N, Cimolai T (2007). The cranberry and the urinary tract. Eur. J. Clin. Microbiol. 26: 767-776. 18- Jass J, Reid G (2009). Effect of cranberry drink on bacterial adhesion in vitro and vaginal microbiota in healthy females. Can. J. Urol. 16: 4901-4907.
18. Jepson RG, Craig JC (2008). Cranberries for preventing urinary tract infections. Cochrane Database Syst. Rev. 23: 2-8.
19. Harkins KJ (2000) what’s the use of cranberry juice? Age and Ageing, 29: 9- 12.
20. Raz R, Chazan B, Dan M (2004). Cranberry Juice and Urinary infections.Clin. Inf. Dis., 38: 1413-1419.
21. Johnson BJ, Lin B, Rubin RA, Malanoski AP (2009). Media acidification by Escherichia coli in the presence of cranberry juice., 2(226): 1-4
22. Sobota AE (1984). Inhibtion of bacterial adherence by cranberry juice. Potential use for treatment of urinary tract infections, J. Urol., 131: 1013-1016.
23. Zafriri D, Ofek I, Adar R, Pocino M, Sharon N (1989). Inhibitory activity of cranberry juice on adherence of type I and P fimbriated Escherichia coli to eucaryotic cells. Antimicrob. Agents Chemother. 33: 92-98.