Isolation and Identification of Fungi Contaminated with Some Local and Imported Raisins and the Possibility of Controlling One of the Toxin-Producing Fungi by Using Ganoderma Lucidium Filtrate and Calcium Carbonate Treatments

  • Tayseer J. Khalaf Department of Biology, College of science, Al-Qadisiya University, Al-Diwaniya, Iraq
  • Abdul Ameer S. Saadon Department of Biology, College of science, Al-Qadisiya University, Al-Diwaniya, Iraq
Keywords: Contamination food, Raisins, Fungal toxins, Aspergillus tubingensis, Ganoderma lucidium

Abstract

Samples were collected from the shops, and local markets in the city of Al- Diwaniyah for the period from (October – December 2022) and multiple species of fungi were isolated: Aspergillus niger, Aspergillus flavus, Aspergillus tubingensis, Aspergillus teereus, Penicillium natatum, Rhizopu stolanifer, Alternaria Alternata, Fusarium sp, Some significant differences were observed in the fungi isolated from raisins and currant the frequency percentage highest in the treatment of non-sterilized from sterilized. A.tubingensis diagnosed phenotypically using classification keys and molecularly diagnosed by PCR technique (polymerase chain reaction). The results of the HPLC technique observed that the highest concentration of Aflatoxin B1 was due to the filterate of Aspergillus tubingensis isolate of the currant sample, which equalize to 114.9 ppb. Ganoderma lucidium filtrate and calcium carbonate shown their potential to significantly reduce the radial activity and dry weight of the this fungal isolate, where the maximum inhibition rate was76.85% for dry weight for radial activity at 30% concentration to treated the interplay between G.lucidium filtrate and calcium carbonate, while the highest inhibition rate was76.66 % for dry weight at 30% for same treat.

References

1. Akiyma , H. and Chen,D.Y. (1999).Simle HPLC determination of aflatoxin B1.B2.G1.G2. in nut and corn , J. of food Hygienicscocity of Japan. 37 (4): 195-201.
2. Al- Saidi, Ghaleb Hussein. (2018). Evaluation of the role of Pleurotus ostreatus and CaCO3 in controlling Alternaria alternata isolated from somelocal and imported food products in Al-Diwaniyah city. Master’s thesis. College of Science - University of Al-Qadisiyah.
3. Al- Shibli, Majed KazemAbbod .Biological resistance of pathogenic fungi and secondary fungi associated (1998).Master thesis . Faculty of Education . Al– Qadisiyah University.
4. Al-Ameri, W., Teklu, T., Graves, R., Kazemi, H., &AlSumaiti, A. (2015, April). Low- salinity water-alternate-surfactant in Low-permeability Carbonate Reservoirs. In IOR 2015-18th European Symposium on Improved Oil Recovery (pp. cp-445). European Association of Geoscientists & Engineers.
5. Alghalibi, S.M. and A.R. Shater. (2004). Mycoflora and mycotoxin contamination of some dried fruits in Yemen Republic. Ass. Uni. Bull. Environ. Res., 7: 19-27.
6. Al-Saidi, Abbas Jabbar. (2013). Phenotypic and molecular characterization of some fungi accompanying wheat seeds and roots and the effect of some pesticides and chemical salts on the growth of these fungi. Master Thesis. College of Science - University of Al-Qadisiyah.
7. Bell, D. K.Well, H.O. and Markham, C. R. (1982). In vitro antagonisms of Trichodermasp. Against six fungeal plant pathogens. Phytopathololgy, 72 p:37-382.
8. Capote, N., Pastrana, A. M., Aguado, A., & Sanchez-Torres, P. (2012). Molecular tools for detection of plant pathogenic fungi and fungicide resistance. Plant Pathology, 151–202.
9. Chowdhury, S. P., Hartmann, A., Gao, X., &Borriss, R. (2015). Biocontrol mechanism by root-associated Bacillus amyloliquefaciens FZB42–a review.Frontiers in microbiology, 6, 780 .
10. Collemare, J., Billard, A., Bohnert, H. U., & Lebrun, M.-H. (2008) Biosynthesis of secondary metabolites in the rice blast fungus Magnaporthe grisea: the role of hybrid PKS-NRPS in pathogenicity. Mycological Research, 112(2), 207–215. .
11. Dixit, S. N.; Tripathy, S. C. and upadyyey, R.R. (1976). The antifungal substasnce of rose flower (Rose indica) Economic Botany. 30:371-373 .
12. Esterla, C. R. A.; Barbin, E.L. ;Spano, J.C.; Marchesan, M.A.& pecora,J.D.(2002). Mechanism of Action of Sodium hypochlorite Braz.Dent. J., 13(2): 113-117 Ethnopharmacology;101(1-3):116-9.
13. Gregori A. S. Vagils, M. and Pohleven J. (2007). Cultivation Tecgnique and Medicinal propertie of Pleurotus spp. Food Technol. Biotechnol., 45: 238-249.
14. Makun, H. A., S. T. Anjorin, B.Moronfoye, F. O. Adeje, O. A. Afolablil, G. Fagbayibo, B.O. Balogum and Surajudeen A.A.(2010). Fungal and aflatoxin contamination of me human food commodities in nigeria. Afiican Journal of Food Science , 4:127 -135.
15. Mikusova, P., Cabon, M., Melicharkova, A., Urík, M., Ritieni, A., & Slovak, M.(2020). Genetic diversity, Ochratoxin A and fumonisin profiles of strains of Aspergillus Section Nigri isolated from dried vine fruits. Toxins, 12(9), 592.
16. Mills, A. A. S., Platt, H. W., and Hurta, R. A. (2004). Effect of salt compounds on mycelial growth, sporulation and spore germination of various potato pathogens. Postharvest Biology and Technology, 34(3), 341-350.
17. Moller, T. E., & Nyberg, M. (2003). Ochratoxin A in raisins and currants: basic extraction procedure used in two small marketing surveys of the occurrence and control of the heterogeneity of the toxins in samples. Food Additives and Contaminants, 20(11), 1072-1076.
18. Palumbo, J. D., O'KEEFFE, T. L., Ho, Y. S., & Santillan, C. J.(2015). Occurrence of ochratoxin A contamination and detection of ochratoxigenic Aspergillus species in retail samples of dried fruits and nuts. Journal of Food Protection, 78(4), 836-842.
19. Park, D. L.; NjapauH. andBoutrifE. (2009). Minimizing risks posed by mycotoxins utilizing the HACCP councept.
20. Schoch, C.L.; Seifert, KAHuhndorf, S.; Robert, V.; Spouge, J.L.,Levesque, C.A. Chen, W. (2012). Nuclear ribosomal internal transcribed spacer (ITS) region as a universal DNA barcode marker for fungi. Proc Natl Acad Sci U S A 109:6241-6246
21. Smith, J. E. (2020). Aflatoxins. In Handbook of plant and fungal toxicants (pp. 269-285). CRC Press.
22. Webster, J., & Weber, R. (2007). Introduction to fungi. Cambridge university press.
Published
2023-06-08
How to Cite
Tayseer J. Khalaf, & Abdul Ameer S. Saadon. (2023). Isolation and Identification of Fungi Contaminated with Some Local and Imported Raisins and the Possibility of Controlling One of the Toxin-Producing Fungi by Using Ganoderma Lucidium Filtrate and Calcium Carbonate Treatments. Central Asian Journal of Medical and Natural Science, 4(3), 573-581. https://doi.org/10.17605/cajmns.v4i3.1567
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Articles