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Abstract

Background: Due to the emergence of multiple antibiotic resistance by P. mirabilis bacteria, it was necessary to search for alternatives other than antibiotics to eliminate these bacteria, and this is what prompted scientists to use nanoparticles, which are nano-size materials that have a significant impact on the components of the bacterial cell. Among these nanomaterials are zinc oxides, titanium oxides, copper, cobalt, silver and gold.Objectives: The study of effect of nanoparticles on the gene expression of some swarming genes in Proteus mirabilis bacteria.Methods: A total of 30 samples collected from urinary tract infections and wounds in sterile containers in different hospitals in Baquba city (Baquba Teaching Hospital and Al-Batool Hospital) were subjected to Molecular detection about wosA and rsbA genes and Detecting the effect of nanoparticles on gene expression of gene wosA and rsbA .


Results: Twenty isolates were obtained, all of which had wosA and rsbA genes . Two isolates of urine and burns were selected to determine the effect of nanomaterials on gene expression of the wosA and rsbA genes encoding for swarming. The results of the test of the effect of silver and zinc oxide nanoparticles on the expression of the rsbA and wosA genes responsible for the phenomenon of swarming showed a decrease in gene expression for isolates after treatment compared to pre-treatment gene expression and by 1 before treatment and 0.44 after treatment for the rsbA gene, while for wosA gene, the gene expression of isolates decreased, as the expression before treatment 1 decreased to 0.76 after treatment with nanomaterial.


Conclusion: Isolates of Proteus mirabilis bacteria, which have virulence factors, it causes many diseases and susceptible to infection through their ability to move, transfer, adhere, and form a biofilm.

Keywords

Proteus mirabilis wosA rsbA gene gene expression nanoparticle

Article Details

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Ibraheem M. AL-Dulaimy, Hadi R. Rasheed Al-Taai, & Ali Jaffar Saleem. (2023). Effect of Silver and Zinc Oxide Nanoparticles on Gene Expression of Some Swarming Genes in Proteus Mirabilis. Central Asian Journal of Medical and Natural Science, 4(3), 11-20. Retrieved from https://cajmns.centralasianstudies.org/index.php/CAJMNS/article/view/1480
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