The Effect of Cronobacter Sakazakii on the Gastro-Intestinal Tract of Newborn Mice

Hayder A.  Al-Mandalawii; Estabraq A.  Mahmoud; Luma Abdulhady  Zwain

doi:10.17605/cajmns.v4i1.1315

Hayder A. Al-Mandalawii Directorate of education of Al-Rasafa/ Ministry of Education, Baghdad, Iraq
Estabraq A. Mahmoud Department of Biology /Collage of Education Pure Science Ibn Al-Haitham/University of Baghdad, Baghdad, Iraq
Luma Abdulhady Zwain Department of Biology /Collage of Education Pure Science Ibn Al-Haitham/University of Baghdad, Baghdad, Iraq

DOI: https://doi.org/10.17605/cajmns.v4i1.1315

Keywords: Animal models, Cronobacter sakazakii, Gastro-intestinal tract, Mice, Newborn

Abstract

Fifteenth of Cronobacter sakazakii were obtained from previous studies isolates were (5 infant formula, 5 spinal fluid and 5 bloods). C. sakazakii is ability to survive on long time in environment as powder infant material which leads transmission bacteria to the immune compromised infant . Fifteen new born mice were obtained from the Iraqi Center for Cancer and Medical Genetics whose ages ranged between 10-17 days and their weights 8.5 ± 1.25 gm, were divided into 3 groups. The samples were cut and placed in formalin, then placed in stabilizer and concentrations of alcohol (70%, 80%, 90%,100%) to complete dehydration after they were leached with xylene, embedded the samples paraffin wax (58°C) and sectioning the with thickness (5) micrometer and stained with Haematoxylin and Eosin stain. The results showed that swelled of the gastro-intestinal tract and fluid accumulation was observed for the animals vaccinated with C. sakazakii an increase in the weight of the gastro-intestinal tract for the mice compared to the control treatment and this increase was significant at the probability level p≤0.05 this may be due to the occurrence of inflammatory symptoms in the affected organ 72 hours after the injury the histological study of the gastro-intestinal tract and the tunica mucosa was distinguished by deep enterocytes goblet cells and muscular was thick . It was represented by loss of villi shedding of the epithelium with rupture of the intestinal epithelial cells and their rupture in the lower of the gastrointestinal tract. Edema of the sub mucosal epithelium has been observed. Goblet cells necrosis and filter immune cells neutrophils, macrophages, eosinophil, and lymphocytes fill the lumen C. sakazakii concentration 103 and 105 cells necrotizing enterocolitis of the newborn mouse.

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